PMID- 11738870
OWN - NLM
STAT- MEDLINE
DCOM- 20020313
LR  - 20220331
IS  - 0387-7604 (Print)
IS  - 0387-7604 (Linking)
VI  - 23 Suppl 1
DP  - 2001 Dec
TI  - Multicolor fluorescent in situ hybridization on post-mortem brain in 
      schizophrenia as an approach for identification of low-level chromosomal 
      aneuploidy in neuropsychiatric diseases.
PG  - S186-90
AB  - Fluorescence in situ hybridization (FISH) of DNA-DNA or DNA-RNA using post-mortem 
      brain samples is one approach to study low-level chromosomal aneuploidy and 
      selective expression of specific genes in the brain of patients with 
      neuropsychiatric diseases. We have performed a pilot molecular-cytogenetic 
      analysis of post-mortem brain of schizophrenic patients. Multicolor FISH on two 
      post-mortem brain samples of normal individuals and six schizophrenic individuals 
      (area 10 of cortex) was applied. A set of DNA probes for FISH included: (i) 
      centromeric alphoid DNA probes for chromosomes 7, 8, 13 and 21, 18, X and Y; (ii) 
      classical satellite DNA probes for chromosomes 1 and 16; and (iii) 
      region-specific DNA probes for chromosomes 13, 21 and 22. A statistically 
      significant level of aneuploidy (up to 0.5-4% of neurons) involving chromosomes X 
      and 18 was detected in two post-mortem brains of patients with schizophrenia. 
      These results indicate that low-level chromosomal aneuploidy could be involved in 
      the pathogenesis of schizophrenia. FISH could be applied to extended studies of 
      chromosomal aneuploidy, abnormal patterns of chromosomal organization and 
      functional gene expression in situ in the neurons of the brain in different 
      psychiatric and neurodevelopmental diseases. Schizophrenia and Rett syndrome 
      might be considered as psychiatric diseases of special interest for 
      molecular-cytogenetic analysis as both of them could be associated with mutations 
      in genes involving regulation of neurodevelopmental processes in the brain.
FAU - Yurov, Y B
AU  - Yurov YB
AD  - National Research Center of Mental Health, Russian Academy of Medical Sciences, 
      113152, Zagorodnoe sh. 2, Moscow, Russia. yurov@rcmh.msk.ru
FAU - Vostrikov, V M
AU  - Vostrikov VM
FAU - Vorsanova, S G
AU  - Vorsanova SG
FAU - Monakhov, V V
AU  - Monakhov VV
FAU - Iourov, I Y
AU  - Iourov IY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Dev
JT  - Brain & development
JID - 7909235
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Aneuploidy
MH  - Brain/*metabolism/pathology/physiopathology
MH  - DNA Mutational Analysis/*methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Mutation/*genetics
MH  - Pilot Projects
MH  - Postmortem Changes
MH  - Schizophrenia/*genetics/*pathology
MH  - Sex Chromosome Aberrations
MH  - Sex Chromosomes/genetics
MH  - Sex Factors
EDAT- 2001/12/12 10:00
MHDA- 2002/03/14 10:01
CRDT- 2001/12/12 10:00
PHST- 2001/12/12 10:00 [pubmed]
PHST- 2002/03/14 10:01 [medline]
PHST- 2001/12/12 10:00 [entrez]
AID - S0387760401003631 [pii]
AID - 10.1016/s0387-7604(01)00363-1 [doi]
PST - ppublish
SO  - Brain Dev. 2001 Dec;23 Suppl 1:S186-90. doi: 10.1016/s0387-7604(01)00363-1.