PMID- 11739111
OWN - NLM
STAT- MEDLINE
DCOM- 20020122
LR  - 20171213
IS  - 1931-857X (Print)
IS  - 1522-1466 (Linking)
VI  - 282
IP  - 1
DP  - 2002 Jan
TI  - Enhanced glomerular permeability to macromolecules in the Nagase analbuminemic 
      rat.
PG  - F45-50
AB  - Plasma albumin restricts capillary water filtration. Accordingly, the glomerular 
      ultrafiltration coefficient is higher in Nagase analbuminemic rats (NAR) than in 
      Sprague-Dawley controls. We investigated whether the glomerular permeability to 
      macromolecules is also enhanced in NAR. SDS-PAGE fractionation of urine proteins 
      showed several bands with molecular masses between 60 and 90 kDa in NAR only. 
      Acute administration of BSA to NAR led to nearly complete disappearance of these 
      proteins from urine, an effect partially reversed when most of the exogenous 
      albumin was cleared from circulation. The fractional clearance of 70-kDa dextran 
      was increased in NAR, indicating a size defect. Binding of cationized ferritin to 
      the glomerular basement membrane was decreased in NAR, suggesting associated 
      depletion of fixed anions. The magnitude of cationic ferritin binding correlated 
      negatively with the fractional clearance of 70-kDa dextran, suggesting that the 
      two abnormalities may share a common pathogenic mechanism. Collectively, these 
      results suggest enhanced glomerular permeability to macromolecules in NAR. 
      Albumin may be necessary to maintain the normal glomerular permselectivity 
      properties.
FAU - Fujihara, Clarice Kazue
AU  - Fujihara CK
AD  - Renal Division, Department of Clinical Medicine, Faculty of Medicine, University 
      of Sao Paulo, Sao Paulo 01246-903, Brazil.
FAU - Arcos-Fajardo, Michelle
AU  - Arcos-Fajardo M
FAU - Brandao De Almeida Prado, Euthymia
AU  - Brandao De Almeida Prado E
FAU - Jose Brandao De Almeida Prado, Maria
AU  - Jose Brandao De Almeida Prado M
FAU - Sesso, Antonio
AU  - Sesso A
FAU - Zatz, Roberto
AU  - Zatz R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (Blood Proteins)
RN  - 0 (Dextrans)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Serum Albumin)
RN  - 9007-73-2 (Ferritins)
RN  - EC 3.2.1.52 (Acetylglucosaminidase)
SB  - IM
MH  - Acetylglucosaminidase/*genetics/*metabolism
MH  - Animals
MH  - Blood Proteins/urine
MH  - Capillary Permeability/physiology
MH  - Dextrans/pharmacokinetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Ferritins/analysis
MH  - Kidney Function Tests
MH  - Kidney Glomerulus/chemistry/*metabolism/ultrastructure
MH  - Macromolecular Substances
MH  - Microscopy, Electron
MH  - Proteinuria/genetics/metabolism
MH  - Rats
MH  - Rats, Mutant Strains
MH  - Rats, Sprague-Dawley
MH  - Serum Albumin/*deficiency/pharmacokinetics
EDAT- 2001/12/12 10:00
MHDA- 2002/01/23 10:01
CRDT- 2001/12/12 10:00
PHST- 2001/12/12 10:00 [pubmed]
PHST- 2002/01/23 10:01 [medline]
PHST- 2001/12/12 10:00 [entrez]
AID - 10.1152/ajprenal.2002.282.1.F45 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2002 Jan;282(1):F45-50. doi: 
      10.1152/ajprenal.2002.282.1.F45.