PMID- 11739746
OWN - NLM
STAT- MEDLINE
DCOM- 20020115
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 22
IP  - 1
DP  - 2002 Jan
TI  - The RNA binding protein nuclear factor 90 functions as both a positive and 
      negative regulator of gene expression in mammalian cells.
PG  - 343-56
AB  - Nuclear factor 90 (NF90) was originally isolated in a complex that binds to the 
      antigen recognition response element (ARRE-2) present in the interleukin-2 
      promoter. To characterize the transcriptional properties of NF90 in mammalian 
      cells, we examined its ability to modulate promoter function in cellular 
      transfection assays. NF90-Gal4 fusion proteins inhibited transcription from the 
      adenovirus major late promoter in a fashion that was dependent on Gal4 targeting. 
      Conversely, NF90 activated the cytomegalovirus immediate-early promoter, to which 
      it was not targeted. These effects required distinct but overlapping domains in 
      the C terminus of NF90, which contains a functional nuclear localization signal 
      and two double-stranded-RNA binding motifs. NF90 is present in cellular complexes 
      together with the NF45 protein. Transfection assays showed that NF45 binds NF90 
      strongly and stimulates its ability to activate but not to inhibit gene 
      expression. This report characterizes NF90 as both a positive and negative 
      regulator of gene expression, depending on the promoter context, and suggests a 
      role for NF45 as a regulator of NF90.
FAU - Reichman, Trevor W
AU  - Reichman TW
AD  - Department of Biochemistry and Molecular Biology, New Jersey Medical School and 
      Graduate School of Biomedical Sciences, University of Medicine and Dentistry of 
      New Jersey, Newark, New Jersey 07013-2714, USA.
FAU - Muniz, Luis C
AU  - Muniz LC
FAU - Mathews, Michael B
AU  - Mathews MB
LA  - eng
GR  - R01 AI034552/AI/NIAID NIH HHS/United States
GR  - R01 AI34552/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (ILF2 protein, human)
RN  - 0 (NFATC Transcription Factors)
RN  - 0 (Nuclear Factor 45 Protein)
RN  - 0 (Nuclear Factor 90 Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Amino Acid Sequence
MH  - Cell Line
MH  - Cytomegalovirus/genetics
MH  - DNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - Gene Expression Regulation/*genetics
MH  - Genes, Reporter/genetics
MH  - Humans
MH  - Luciferases/genetics/metabolism
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - NFATC Transcription Factors
MH  - Nuclear Factor 45 Protein
MH  - Nuclear Factor 90 Proteins
MH  - Nuclear Proteins/chemistry/genetics/metabolism
MH  - Plasmids/genetics
MH  - Promoter Regions, Genetic/*genetics
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sequence Alignment
MH  - Transcription Factors/chemistry/genetics/*metabolism
MH  - Transcription, Genetic/*genetics/physiology
MH  - Transfection
PMC - PMC134226
EDAT- 2001/12/12 10:00
MHDA- 2002/01/16 10:01
PMCR- 2002/01/01
CRDT- 2001/12/12 10:00
PHST- 2001/12/12 10:00 [pubmed]
PHST- 2002/01/16 10:01 [medline]
PHST- 2001/12/12 10:00 [entrez]
PHST- 2002/01/01 00:00 [pmc-release]
AID - 1127 [pii]
AID - 10.1128/MCB.22.1.343-356.2002 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2002 Jan;22(1):343-56. doi: 10.1128/MCB.22.1.343-356.2002.