PMID- 11741947
OWN - NLM
STAT- MEDLINE
DCOM- 20020424
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 8
DP  - 2002 Feb 22
TI  - Brain-derived neurotrophic factor-induced potentiation of Ca(2+) oscillations in 
      developing cortical neurons.
PG  - 6520-9
AB  - Brain-derived neurotrophic factor (BDNF) has been reported to exert an acute 
      potentiation of synaptic activity. Here we examined the action of BDNF on 
      synchronous spontaneous Ca(2+) oscillations in cultured cerebral cortical neurons 
      prepared from postnatal 2-3-day-old rats. The synchronous spontaneous Ca(2+) 
      oscillations began at approximately DIV 5. It was revealed that voltage-dependent 
      Ca(2+) channels and ionotropic glutamate receptors were involved in the 
      synchronous spontaneous oscillatory activity. BDNF potentiated the frequency of 
      these oscillations. The BDNF-potentiated activity reached 207 +/- 20.1% of basal 
      oscillatory activity. NT-3 and NT-4/5 also induced the potentiation. However, 
      nerve growth factor did not. We examined the correlation between BDNF-induced 
      glutamate release and the BDNF-potentiated oscillatory activity. Both 
      up-regulation of phospholipase C-gamma (PLC-gamma) expression and the 
      BDNF-induced glutamate release occurred at approximately DIV 5 when the 
      BDNF-potentiated oscillations appeared. We confirmed that the BDNF-induced 
      glutamate release occurred through a glutamate transporter that was dependent on 
      the PLC-gamma/IP(3)/Ca(2+) pathway. Transporter inhibitors blocked the 
      BDNF-potentiated oscillations, demonstrating that BDNF enhanced the glutamatergic 
      transmissions in the developing cortical network by inducing glutamate release 
      via a glutamate transporter.
FAU - Numakawa, Tadahiro
AU  - Numakawa T
AD  - Division of Protein Biosynthesis, Institute for Protein Research, Osaka 
      University, Suita, Osaka 565-0871, Japan. numakawa@protein.osaka-u.ac.jp
FAU - Yamagishi, Satoru
AU  - Yamagishi S
FAU - Adachi, Naoki
AU  - Adachi N
FAU - Matsumoto, Tomoya
AU  - Matsumoto T
FAU - Yokomaku, Daisaku
AU  - Yokomaku D
FAU - Yamada, Masashi
AU  - Yamada M
FAU - Hatanaka, Hiroshi
AU  - Hatanaka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20011210
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Isoenzymes)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Oncogene Proteins)
RN  - 0 (oncogene protein trk)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - Amino Acid Transport System X-AG/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Calcium Signaling/*drug effects/physiology
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/*physiology
MH  - Glutamic Acid/metabolism/pharmacology
MH  - Isoenzymes/metabolism
MH  - Kinetics
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Neurons/cytology/*drug effects/physiology
MH  - Neurotransmitter Agents/metabolism
MH  - Oncogene Proteins/metabolism
MH  - Oscillometry
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phospholipase C gamma
MH  - Rats
MH  - Type C Phospholipases/metabolism
EDAT- 2001/12/14 10:00
MHDA- 2002/04/25 10:01
CRDT- 2001/12/14 10:00
PHST- 2001/12/14 10:00 [pubmed]
PHST- 2002/04/25 10:01 [medline]
PHST- 2001/12/14 10:00 [entrez]
AID - S0021-9258(19)82436-5 [pii]
AID - 10.1074/jbc.M109139200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Feb 22;277(8):6520-9. doi: 10.1074/jbc.M109139200. Epub 2001 
      Dec 10.