PMID- 11742034
OWN - NLM
STAT- MEDLINE
DCOM- 20020110
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 81
IP  - 12
DP  - 2001 Dec
TI  - Frequent RET rearrangements in thyroid papillary microcarcinoma detected by 
      interphase fluorescence in situ hybridization.
PG  - 1639-45
AB  - Papillary thyroid microcarcinomas (measuring 1 cm or less in diameter) are very 
      common thyroid tumors, which are present in 10% to 35% of post-mortem 
      histopathological examinations of individuals whose death was due to a cause 
      other than thyroid cancer. The molecular basis of this tumor is still poorly 
      understood. Somatic mutations are better characterized in clinically evident 
      papillary thyroid carcinomas (PTCs), the most common involving the proto-oncogene 
      RET, which maps to 10q11.2. Molecular alterations of RET always lead to intra- or 
      interchromosomal rearrangements. In this study we have investigated the status of 
      RET in 21 microcarcinomas, by means of interphase fluorescence in situ 
      hybridization (FISH). RET was rearranged in 52% of microcarcinomas, a 
      statistically significant higher frequency than that found previously in 
      clinically evident PTCs using the same technique. Moreover, interphase FISH 
      allowed us to detect a putative novel type of rearrangement in a microcarcinoma, 
      and we observed trisomies of chromosome 10 and other chromosomes in two adenomas 
      surrounding two of the microcarcinomas. The strikingly high frequency of RET 
      rearrangements in microcarcinomas strongly suggests that RET plays a role in the 
      initiation of thyroid tumorigenesis but does not seem to be necessary for the 
      further progression of the tumor.
FAU - Corvi, R
AU  - Corvi R
AD  - European Center for the Validation of Alternative Methods (RC), Institute for 
      Health and Consumer Protection, Ispra, Italy. corvi@iarc.fr
FAU - Martinez-Alfaro, M
AU  - Martinez-Alfaro M
FAU - Harach, H R
AU  - Harach HR
FAU - Zini, M
AU  - Zini M
FAU - Papotti, M
AU  - Papotti M
FAU - Romeo, G
AU  - Romeo G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Drosophila Proteins)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-ret)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Ret protein, Drosophila)
SB  - IM
MH  - Adult
MH  - Carcinoma/genetics/pathology
MH  - Carcinoma, Papillary/*genetics/pathology
MH  - *Drosophila Proteins
MH  - Female
MH  - Gene Frequency
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins c-ret
MH  - Receptor Protein-Tyrosine Kinases/*genetics
MH  - Thyroid Neoplasms/*genetics/pathology
EDAT- 2001/12/14 10:00
MHDA- 2002/01/11 10:01
CRDT- 2001/12/14 10:00
PHST- 2001/12/14 10:00 [pubmed]
PHST- 2002/01/11 10:01 [medline]
PHST- 2001/12/14 10:00 [entrez]
AID - S0023-6837(22)03132-4 [pii]
AID - 10.1038/labinvest.3780377 [doi]
PST - ppublish
SO  - Lab Invest. 2001 Dec;81(12):1639-45. doi: 10.1038/labinvest.3780377.