PMID- 11742806
OWN - NLM
STAT- MEDLINE
DCOM- 20020115
LR  - 20201208
IS  - 0363-6143 (Print)
IS  - 0363-6143 (Linking)
VI  - 282
IP  - 1
DP  - 2002 Jan
TI  - Two-way arginine transport in human endothelial cells: TNF-alpha stimulation is 
      restricted to system y(+).
PG  - C134-43
AB  - Human umbilical vein endothelial cells transport arginine through two 
      Na(+)-independent systems. System y(+)L is insensitive to N-ethylmaleimide (NEM), 
      inhibited by L-leucine in the presence of Na(+), and referable to the expression 
      of SLC7A6/y(+)LAT2, SLC7A7/y(+)LAT1, and SLC3A2/4F2hc. System y(+) is referable 
      to the expression of SLC7A1/CAT1 and SLC7A2/CAT2B. Tumor necrosis factor-alpha 
      (TNF-alpha) and bacterial lipopolysaccharide induce a transient stimulation of 
      arginine influx and efflux through system y(+). Increased expression of 
      SLC7A2/CAT2B is detectable from 3 h of treatment, while SLC7A1 expression is 
      inhibited at later times of incubation. System y(+)L activity and expression 
      remain unaltered. Nitric oxide synthase type 2 mRNA is not detected in the 
      absence or presence of TNF-alpha, while the latter condition lowers nitric oxide 
      synthase type 3 expression at the mRNA and the protein level. Nitrite 
      accumulation is comparable in cytokine-treated and control cells up to 48 h of 
      treatment. It is concluded that modulation of endothelial arginine transport by 
      TNF-alpha or lipopolysaccharide occurs exclusively through changes in CAT2B and 
      CAT1 expression and is dissociated from stimulation of nitric oxide production.
FAU - Sala, Roberto
AU  - Sala R
AD  - Dipartimento di Medicina Sperimentale, Sezione di Patologia Generale e Clinica, 
      Universita degli Studi di Parma, 43100 Parma, Italy.
FAU - Rotoli, Bianca Maria
AU  - Rotoli BM
FAU - Colla, Emanuela
AU  - Colla E
FAU - Visigalli, Rossana
AU  - Visigalli R
FAU - Parolari, Alessandro
AU  - Parolari A
FAU - Bussolati, Ovidio
AU  - Bussolati O
FAU - Gazzola, Gian C
AU  - Gazzola GC
FAU - Dall'Asta, Valeria
AU  - Dall'Asta V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Amino Acid Transport System y+)
RN  - 0 (Amino Acid Transport System y+L)
RN  - 0 (Amino Acid Transport Systems, Basic)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cationic Amino Acid Transporter 1)
RN  - 0 (Cationic Amino Acid Transporter 2)
RN  - 0 (DNA Primers)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fusion Regulatory Protein 1, Light Chains)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nitrites)
RN  - 0 (SLC7A7 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 156320-25-1 (Slc7a2 protein, mouse)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - O3C74ACM9V (Ethylmaleimide)
SB  - IM
MH  - Amino Acid Transport System y+/*physiology
MH  - Amino Acid Transport System y+L
MH  - *Amino Acid Transport Systems, Basic
MH  - Antineoplastic Agents/*pharmacology
MH  - Arginine/*metabolism
MH  - Biological Transport/drug effects/physiology
MH  - Carrier Proteins/genetics/metabolism
MH  - Cationic Amino Acid Transporter 1/genetics/metabolism
MH  - Cationic Amino Acid Transporter 2/genetics/metabolism
MH  - Cells, Cultured
MH  - DNA Primers
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Ethylmaleimide/pharmacology
MH  - Fusion Regulatory Protein 1, Light Chains/genetics/metabolism
MH  - Gene Expression/drug effects/physiology
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Membrane Proteins/genetics/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase/genetics
MH  - Nitric Oxide Synthase Type II
MH  - Nitrites/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Umbilical Veins/cytology
EDAT- 2001/12/18 10:00
MHDA- 2002/01/16 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/01/16 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 10.1152/ajpcell.2002.282.1.C134 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2002 Jan;282(1):C134-43. doi: 
      10.1152/ajpcell.2002.282.1.C134.