PMID- 11744619
OWN - NLM
STAT- MEDLINE
DCOM- 20020403
LR  - 20131121
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 16
IP  - 2
DP  - 2002 Feb
TI  - Ca2+ modulation of volume-regulated anion channels: evidence for colocalization 
      with store-operated channels.
PG  - 222-4
AB  - Ca2+ regulation of Cl- current induced by cell swelling (I(CI,swell)) in response 
      to hypotonicity was studied in human prostate cancer epithelial cells (LNCaP) by 
      using the patch-clamp technique. Increase of global intracellular Ca2+ ([Ca2+]in) 
      to 1 mM as well as variations of the extracellular Ca2+ ([Ca2+]out) in the 0 to 
      10 mM range did not affect time course of the development, maximal amplitude, 
      rectification properties, and kinetics of I(CI,swell). However, the presence of 
      0.1 mM thapsigargin (TG), an inhibitor of endoplasmic reticulum (ER) Ca2+ pump, 
      resulted in a more than 50% inhibition of ICI,swell. The blockade of plasma 
      membrane store-operated channels (SOCs), activated in the presence of TG, by 2 mM 
      Ni2+ prevented TG-conferred I(CI,swell) inhibition by extracellular Ca2+. In the 
      presence of TG and Ca2+, the cells failed to exhibit regulatory volume decrease. 
      We conclude that interaction between volume-regulated anion channels (VRACs) 
      carrying I(CI,swell) and Ca2+ occurs in the microdomains from the inner surface 
      of the membrane that are not accessible to the changes in [Ca2+]in, but can be 
      readily reached by Ca2+ entering the cell via plasma membrane, especially through 
      SOCs. Preferred access of SOC-transported Ca2+ to VRAC suggests colocalization of 
      these channels in the cell membrane.
FAU - Lemonnier, Loic
AU  - Lemonnier L
AD  - Laboratoire de Physiologie Cellulaire, INSERM EPI 9938, Batiment SN3, USTL, 59655 
      Villeneuve d'Ascq, France.
FAU - Prevarskaya, Natalia
AU  - Prevarskaya N
FAU - Shuba, Yaroslav
AU  - Shuba Y
FAU - Vanden Abeele, Fabien
AU  - Vanden Abeele F
FAU - Nilius, Bernd
AU  - Nilius B
FAU - Mazurier, Joel
AU  - Mazurier J
FAU - Skryma, Roman
AU  - Skryma R
LA  - eng
PT  - Journal Article
DEP - 20011214
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Calcium Channels)
RN  - 0 (Chloride Channels)
RN  - 0 (Hypotonic Solutions)
RN  - 67526-95-8 (Thapsigargin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/metabolism/*pharmacology
MH  - Calcium Channels/physiology
MH  - Chloride Channels/*physiology
MH  - Humans
MH  - Hypotonic Solutions/pharmacology
MH  - Male
MH  - Membrane Potentials/drug effects
MH  - Patch-Clamp Techniques
MH  - Thapsigargin/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2001/12/18 10:00
MHDA- 2002/04/04 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/04/04 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 01-0383fje [pii]
AID - 10.1096/fj.01-0383fje [doi]
PST - ppublish
SO  - FASEB J. 2002 Feb;16(2):222-4. doi: 10.1096/fj.01-0383fje. Epub 2001 Dec 14.