PMID- 11745215
OWN - NLM
STAT- MEDLINE
DCOM- 20020107
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 92
IP  - 6
DP  - 2001 Sep 15
TI  - Differential expression of menin in various adrenal tumors. The role of menin in 
      adrenal tumors.
PG  - 1393-401
AB  - BACKGROUND: Adrenocortical tumors occur as sporadic tumors, as part of the 
      multiple endocrine neoplasia type 1 (MEN1) syndrome, or as part of other 
      hereditary disorders. MEN1 is a tumor suppressor gene located on chromosome 11q13 
      that encodes a 610-amino acid protein called menin, and plays an important role 
      in the development of MEN1 syndrome. Recent reports indicate that heterozygous 
      germline mutations of this gene are responsible for the disease onset of MEN1. 
      METHODS: To investigate the role of menin in sporadic adrenocortical tumors, the 
      authors examined a series of adrenocortical adenoma cases and a single case of 
      carcinoma and adrenomedulary tumors with the corresponding adjacent tumor tissues 
      using reverse transcriptase-polymerase chain reaction (RT-PCR) for menin mRNA and 
      Western blot analysis for menin protein. Both RNA and protein from these tumors 
      were applied to RT-PCR and Western blot analysis, respectively, although they are 
      not truly quantitative. Primers for RT-PCR were designed to amplify the sequence 
      between exons 2 and 3 of the MEN1 gene. A specific antibody against menin was 
      generated in guinea pigs immunized with the recombinant peptide from the amino 
      acid residues 443-535 of menin made by using glutathione-S-transferase gene 
      fusion. RESULTS: Based on the results of RT-PCR and Western blot analysis, both 
      MEN1 mRNA and menin protein appeared to be highly expressed in Cushing syndrome 
      resulting from adrenocortical adenomas and carcinoma. However, their expression 
      was found to be greatly decreased in primary aldosteronism compared with their 
      expression in Cushing syndrome. Although weak expression of MEN1 mRNA also was 
      detected in pheochromocytoma on RT-PCR, menin expression was not detected in any 
      case of pheochromocytoma by Western blot analysis, possibly due to the lower 
      sensitivity of this assay compared with RT-PCR. Neither MEN1 mRNA nor menin 
      protein was detected in any of the corresponding adjacent tumor tissues examined. 
      CONCLUSIONS: The findings of the current study indicate that menin expression 
      appears to be up-regulated in Cushing syndrome, suggesting that adrenocortical 
      proliferation might be one of the primary lesions in the MEN1 syndrome in which 
      menin might play a significant role in some specific cellular functions.
CI  - Copyright 2001 American Cancer Society.
FAU - Bhuiyan, M M
AU  - Bhuiyan MM
AD  - First Department of Internal Medicine, Kagawa Medical University, Kagawa, Japan.
FAU - Sato, M
AU  - Sato M
FAU - Murao, K
AU  - Murao K
FAU - Imachi, H
AU  - Imachi H
FAU - Namihira, H
AU  - Namihira H
FAU - Ishida, T
AU  - Ishida T
FAU - Takahara, J
AU  - Takahara J
FAU - Miyauchi, A
AU  - Miyauchi A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adenoma/chemistry
MH  - Adolescent
MH  - Adrenal Cortex Neoplasms/chemistry
MH  - Adrenal Gland Neoplasms/*chemistry
MH  - Adrenal Medulla
MH  - Adult
MH  - Aged
MH  - Blotting, Western
MH  - Carcinoma/chemistry
MH  - Cushing Syndrome/etiology/metabolism
MH  - Female
MH  - Humans
MH  - Hyperaldosteronism/etiology/metabolism
MH  - Male
MH  - Middle Aged
MH  - Multiple Endocrine Neoplasia Type 1/chemistry
MH  - Neoplasm Proteins/*analysis/genetics/*physiology
MH  - Pheochromocytoma/chemistry
MH  - Polymerase Chain Reaction
MH  - *Proto-Oncogene Proteins
MH  - RNA, Messenger/analysis
MH  - Up-Regulation
EDAT- 2001/12/18 10:00
MHDA- 2002/01/10 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/01/10 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 10.1002/1097-0142(20010915)92:6<1393::AID-CNCR1462>3.0.CO;2-4 [pii]
AID - 10.1002/1097-0142(20010915)92:6<1393::aid-cncr1462>3.0.co;2-4 [doi]
PST - ppublish
SO  - Cancer. 2001 Sep 15;92(6):1393-401. doi: 
      10.1002/1097-0142(20010915)92:6<1393::aid-cncr1462>3.0.co;2-4.