PMID- 11745550
OWN - NLM
STAT- MEDLINE
DCOM- 20020315
LR  - 20190910
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 59
IP  - 1
DP  - 2002 Jan
TI  - Metabolic heat production as a measure of macrophage response to particles from 
      orthopedic implant materials.
PG  - 166-75
AB  - An in vitro method to gauge metabolic heat response of macrophages (MO) to 
      particulates is described. Whereas the majority of work cited relies on chemical 
      analysis to assess MO response to particles, we have used isothermal 
      microcalorimetry (IMC) for direct continuous measurement of metabolic heat 
      production to gauge the response. IMC is a screening method, in that it ensures 
      that no energy-consuming phagocytic response goes undetected, and that the 
      aggregate metabolic magnitude of the responses is determined. A four-well IMC was 
      used in all microcalorimetric measurements. To accommodate "zero-time" monitoring 
      of the interaction of particles and cells, a set of identical test chambers was 
      constructed for use in the IMC. MOs were injected from outside the IMC onto 
      particles contained in collagen or gelatin on glass coverslips at the bottom of 
      each chamber. IMC runs were performed using MOs only, MOs and lipopolysaccharide 
      (LPS) positive control, and MOs and clean or LPS-bound particles of either 
      high-density polyethylene (HDPE) or cobalt-chrome alloy (CoCr). Total heat 
      produced by the negative controls (MOs alone) was lower than for MO exposure to 
      LPS or particles. The trend was a higher response for LPS-bound HDPE compared 
      with clean HDPE particles, though not significant. In conclusion, our results 
      have shown that IMC can be used to detect the heat associated with the 
      phagocytosis of particulate materials by MOs in vitro.
CI  - Copyright 2001 John Wiley & Sons, Inc.
FAU - Charlebois, S J
AU  - Charlebois SJ
AD  - University of Tennessee-Campbell Clinic, Department of Orthopaedic Surgery, 956 
      Court Avenue, Room A302, Memphis, Tennessee 38163, USA. 
      steven.charlebois@Zimmer.com
FAU - Daniels, A U
AU  - Daniels AU
FAU - Smith, R A
AU  - Smith RA
LA  - eng
GR  - 1R41-AR44581-01/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Bone Substitutes)
SB  - IM
MH  - *Bone Substitutes
MH  - Calorimetry/methods
MH  - Energy Metabolism
MH  - *Hot Temperature
MH  - Macrophages/*physiology
EDAT- 2001/12/18 10:00
MHDA- 2002/03/16 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/03/16 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 10.1002/jbm.1230 [pii]
AID - 10.1002/jbm.1230 [doi]
PST - ppublish
SO  - J Biomed Mater Res. 2002 Jan;59(1):166-75. doi: 10.1002/jbm.1230.