PMID- 11746121
OWN - NLM
STAT- MEDLINE
DCOM- 20020131
LR  - 20190906
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 21
IP  - 10
DP  - 2001 Oct
TI  - Fetal and maternal progenitor cells in co-culture respond equally to 
      erythropoietin.
PG  - 818-23
AB  - To explore potentially selective growth conditions for fetal cells in cultures 
      from the blood of pregnant women, we investigated if fetal and adult erythroid 
      progenitors with different hemoglobin expression programs are differentially 
      responsive to erythropoietin (EPO). Co-cultures of clonogenic cells from 12-week 
      fetal and adult peripheral blood were established, and the development of 
      erythropoietic cells was monitored using flow cytometric profiles of correlated 
      cellular contents of fetal and adult hemoglobin (HbF and HbA, respectively). 
      Adult nucleated red cells were classified as F+A-, F+A+ or F-A+. All fetal cells 
      were F+A-. The population of F+A- cells was flow-sorted and fetal cells were 
      identified by fluorescence in situ hybridization (FISH) using chromosome-specific 
      probes. Delayed EPO addition revealed that all types of erythroid cells entered 
      the EPO-dependent phase with similar kinetics, beginning at about Day 4. The data 
      suggest that fetal and adult erythroid stem/progenitor cells have the same 
      initial maturation kinetics in culture independent of their hemoglobin chain 
      expression program. Fetal and adult cells with different hemoglobin profiles also 
      showed similar EPO dose-response curves, determined for different intervals 
      during the first 2 weeks of culture. Thus, the kinetics of entry into the phase 
      of EPO dependence, as well as the sensitivity to EPO at various stages of 
      development, are essentially the same for erythropoietic progenitor cells derived 
      from adult and early fetal blood, which rules out the possibility of using the 
      timing or concentration of EPO for the selective growth of fetal cells from the 
      blood of pregnant women.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Bohmer, R M
AU  - Bohmer RM
AD  - Division of Genetics, Department of Pediatrics, Tufts University School of 
      Medicine, Boston, MA, USA. rbohmer@lifespan.org
FAU - Johnson, K L
AU  - Johnson KL
FAU - Bianchi, D W
AU  - Bianchi DW
LA  - eng
PT  - Journal Article
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
RN  - 11096-26-7 (Erythropoietin)
RN  - 9034-51-9 (Hemoglobin A)
RN  - 9034-63-3 (Fetal Hemoglobin)
SB  - IM
MH  - Coculture Techniques
MH  - Erythroid Precursor Cells/chemistry/*cytology/drug effects
MH  - Erythropoiesis
MH  - Erythropoietin/*pharmacology
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Fetal Hemoglobin/analysis
MH  - Flow Cytometry
MH  - Gestational Age
MH  - Hemoglobin A/analysis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Pregnancy
EDAT- 2001/12/18 10:00
MHDA- 2002/02/01 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/02/01 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 10.1002/pd.144 [pii]
AID - 10.1002/pd.144 [doi]
PST - ppublish
SO  - Prenat Diagn. 2001 Oct;21(10):818-23. doi: 10.1002/pd.144.