PMID- 11746158
OWN - NLM
STAT- MEDLINE
DCOM- 20020301
LR  - 20190906
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 21
IP  - 12
DP  - 2001 Dec
TI  - Significantly higher number of fetal cells in the maternal circulation of women 
      with pre-eclampsia.
PG  - 1022-6
AB  - Although the pathophysiology of pre-eclampsia is unknown, several studies have 
      indicated that abnormal placentation early in pregnancy might play a key role. It 
      has recently been suggested that this abnormal placentation may result in 
      transfusion of fetal cells (feto-maternal transfusion) in women with 
      pre-eclampsia. In the present study, fetal nucleated red blood cells were 
      isolated from 20 women with pre-eclampsia and 20 controls using a very efficient 
      magnetic activated cell sorting (MACS) protocol. The number of male cells was 
      determined using two-color fluorescence in situ hybridization (FISH) for X and Y 
      chromosomes. Significantly more XY cells could be detected in women with 
      pre-eclampsia (0.61+/-1.2 XY cells/ml blood) compared to women with uncomplicated 
      pregnancies (0.02+/-0.04 XY cells/ml blood) (Mann-Whitney U-test, p<0.001). These 
      results suggest that fetal cell trafficking is enhanced in women with 
      pre-eclampsia, and this finding may contribute to the understanding of the 
      pathophysiology of the disease.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Jansen, M W
AU  - Jansen MW
AD  - Department of Obstetrics and Gynecology, Erasmus University and University 
      Hospital Dijkzigt, Rotterdam, The Netherlands. jansen@ikg.fgg.eur.nl
FAU - Korver-Hakkennes, K
AU  - Korver-Hakkennes K
FAU - van Leenen, D
AU  - van Leenen D
FAU - Visser, W
AU  - Visser W
FAU - in 't Veld, P A
AU  - in 't Veld PA
FAU - de Groot, C J
AU  - de Groot CJ
FAU - Wladimiroff, J W
AU  - Wladimiroff JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - Cell Separation
MH  - Erythrocyte Count
MH  - Erythrocytes
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Fetomaternal Transfusion/complications
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Magnetics
MH  - Male
MH  - Pre-Eclampsia/*blood/etiology
MH  - Pregnancy
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 2001/12/18 10:00
MHDA- 2002/03/02 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/03/02 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 10.1002/pd.176 [pii]
AID - 10.1002/pd.176 [doi]
PST - ppublish
SO  - Prenat Diagn. 2001 Dec;21(12):1022-6. doi: 10.1002/pd.176.