PMID- 11746357
OWN - NLM
STAT- MEDLINE
DCOM- 20020125
LR  - 20161124
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 66
IP  - 3
DP  - 2001 Nov 1
TI  - Induction and axonal localization of epithelial/epidermal fatty acid-binding 
      protein in retinal ganglion cells are associated with axon development and 
      regeneration.
PG  - 396-405
AB  - Epithelial/epidermal fatty acid-binding protein (E-FABP) is induced in peripheral 
      neurons during nerve regeneration and is found at high levels in central neurons 
      during neuronal migration and development. Furthermore, E-FABP expression is 
      required for normal neurite outgrowth in PC12 cells treated with nerve growth 
      factor (NGF). The present study examined whether E-FABP plays a role in retinal 
      ganglion cell (RGC) differentiation and axon growth. Rat retinal tissues from 
      embryonic (E) and postnatal (P) development through adulthood were examined using 
      immunocytochemical labeling with E-FABP and growth-associated protein 43 (GAP-43) 
      antibodies. E-FABP colocalized with GAP-43 at E14 through P10. At E14, E-FABP 
      immunoreactivity was confined to the somas of GAP-43-positive cells in the 
      ganglion cell layer, but it was localized to their axons by E15. The axons in the 
      optic nerve were GAP-43-positive and E-FABP-negative on E15, but the two proteins 
      were colocalized by E18. Retinal cultures at E15 confirmed that E-FABP and GAP-43 
      colocalize in RGCs. Postnatally, labeling was present between P1 and P10 but 
      decreased at older ages and was minimally present or absent in adult animals. 
      Western immunoblotting revealed that at E18, P1, and P10 E-FABP levels were at 
      least fourfold greater than those in the adult. By P15, protein levels were only 
      twofold greater, with adult levels reached by P31. Furthermore, E-FABP could be 
      reinduced during axon regeneration. Dissociated P15 retinal cells cultured in the 
      presence of brain-derived neurotrophic factor, ciliary neurotrophic factor, and 
      basic fibroblast growth factor exhibited sixfold more GAP-43 and E-FABP 
      double-positive RGCs (cell body and axons) than controls. Moreover, all 
      GAP-43-immunoreactive RGCs were also positive for E-FABP. Taken together, these 
      results indicate the following: 1) E-FABP is expressed in RGCs as they reached 
      the ganglion cell layer and 2) E-FABP plays a functional role in the elaboration 
      of RGC axons in both development and regeneration.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Allen, G W
AU  - Allen GW
AD  - Department of Physiology and Pharmacology, Center for Molecular Biology and Gene 
      Therapy, Loma Linda University, Loma Linda, California 92350, USA.
FAU - Liu, J
AU  - Liu J
FAU - Kirby, M A
AU  - Kirby MA
FAU - De Leon, M
AU  - De Leon M
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Carrier Proteins)
RN  - 0 (Fabp7 protein, rat)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Tubulin)
SB  - IM
MH  - Aging/drug effects/physiology
MH  - Animals
MH  - Animals, Newborn
MH  - Antibody Specificity
MH  - Carrier Proteins/*metabolism/pharmacology
MH  - Cell Differentiation/drug effects/*physiology
MH  - Cell Movement/drug effects/physiology
MH  - Cell Survival/drug effects/physiology
MH  - Cells, Cultured/cytology/drug effects/metabolism
MH  - Embryonic Induction/drug effects/*physiology
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Female
MH  - Fetus
MH  - GAP-43 Protein/metabolism
MH  - Growth Cones/drug effects/*metabolism/ultrastructure
MH  - Immunohistochemistry
MH  - *Neoplasm Proteins
MH  - Nerve Regeneration/drug effects/*physiology
MH  - *Nerve Tissue Proteins
MH  - Rats
MH  - Rats, Long-Evans
MH  - Retina/cytology/*embryology/*growth & development
MH  - Retinal Ganglion Cells/cytology/drug effects/*metabolism
MH  - Tubulin/metabolism
MH  - Up-Regulation/drug effects/physiology
EDAT- 2001/12/18 10:00
MHDA- 2002/01/26 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/01/26 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 10.1002/jnr.1232 [pii]
AID - 10.1002/jnr.1232 [doi]
PST - ppublish
SO  - J Neurosci Res. 2001 Nov 1;66(3):396-405. doi: 10.1002/jnr.1232.