PMID- 11746382
OWN - NLM
STAT- MEDLINE
DCOM- 20020124
LR  - 20131121
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 66
IP  - 4
DP  - 2001 Nov 15
TI  - Changes in neurofilament protein-immunoreactivity after kainic acid treatment of 
      organotypic hippocampal slice cultures.
PG  - 620-9
AB  - Neurofilament (NF) proteins are expressed in the majority of neurons in the 
      central nervous system, and play a crucial role in the organization of neuronal 
      shape and function. In the present study, we have used immunoblotting and 
      immunocytochemical methods to study the light (NF-L), medium (NF-M ), and heavy 
      (NF-H) molecular weight NF proteins in cultured organotypic hippocampal slices 
      during the in vitro maturation and the changes after kainic acid (KA) treatment. 
      In control cultures at 11 DIV throughout 25 DIV, CA3 pyramidal neurons and their 
      proximal dendrites were heavily labeled with the antibodies against all three NF 
      proteins. In CA1 pyramidal neurons, no staining was detected in any age group. A 
      few weakly NF-L positive granule cells with fibers were detected in each age 
      group, whereas NF-M and NF-H positive granule cells first appeared in the older 
      cultures. The application of KA (5 microM) to the cultures for 48 hr, induced a 
      pronounced cell death in the CA3 cell layers, and also moderately damaged granule 
      cells. After the treatment, the immunoblot signal of NF-L and NF-M markedly 
      decreased, whereas that of NF-H almost completely disappeared. The amount of NF-L 
      positive fibers, however, dramatically increased in the molecular and hilar 
      regions of the dentate gyrus in both age groups. Our results show the cellular 
      heterogeneity in the distribution of NF protein triplet in cultured organotypic 
      hippocampal slices. Kainic acid treatment induced changes, which mimicked those 
      observed in the hippocampal region of epileptic animals.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Holopainen, I E
AU  - Holopainen IE
AD  - Department of Pharmacology and Clinical Pharmacology, University of Turku, Turku, 
      Finland. irma.holopainen@utu.fi
FAU - Lauren, H B
AU  - Lauren HB
FAU - Romppanen, A
AU  - Romppanen A
FAU - Lopez-Picon, F R
AU  - Lopez-Picon FR
LA  - eng
GR  - NS17771/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Neurotoxins)
RN  - 0 (neurofilament protein L)
RN  - 111365-29-8 (neurofilament protein M)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Aging/drug effects/*physiology
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Death/drug effects/physiology
MH  - Down-Regulation/drug effects/physiology
MH  - Epilepsy/chemically induced/*metabolism/pathology
MH  - Excitatory Amino Acid Agonists/pharmacology
MH  - Hippocampus/growth & development/*metabolism/pathology
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Kainic Acid/pharmacology
MH  - Neurofilament Proteins/drug effects/*metabolism
MH  - Neuronal Plasticity/drug effects/*physiology
MH  - Neurotoxins/pharmacology
MH  - Organ Culture Techniques
MH  - Pyramidal Cells/drug effects/*metabolism/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Up-Regulation/drug effects/physiology
EDAT- 2001/12/18 10:00
MHDA- 2002/01/25 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/01/25 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 10.1002/jnr.10005 [pii]
AID - 10.1002/jnr.10005 [doi]
PST - ppublish
SO  - J Neurosci Res. 2001 Nov 15;66(4):620-9. doi: 10.1002/jnr.10005.