PMID- 11746962
OWN - NLM
STAT- MEDLINE
DCOM- 20020212
LR  - 20041117
IS  - 1040-452X (Print)
IS  - 1040-452X (Linking)
VI  - 60
IP  - 4
DP  - 2001 Dec
TI  - Acrocentric centromere organization within the chromocenter of the human sperm 
      nucleus.
PG  - 507-16
AB  - It has recently been reported that in human sperm cells, the centromeres are 
      clustered in a chromocenter in the interior region of the nucleus. The aim of the 
      present study was to determine the intra-chromocenter organization of the five 
      centromeres of the acrocentric chromosomes responsible for the biosynthesis of 
      rRNA. The acrocentric centromeres were labeled by fluorescence in situ 
      hybridization (FISH) after mild decondensation of the sperm nuclei to preserve 
      the tail structure. The tail was used as a topographical marker for the 
      orientation of the nucleus. The following results were obtained: (a) the 
      association among the five centromeres was higher than expected from random 
      distribution; (b) all the centromeres observed were randomly located within the 
      chromocenter, occupying about 87% of the total area of the internal nucleus; (c) 
      a major subpopulation of centromeres was located in a preferred area occupying 
      8.3% of the total nuclear area, with a peak 0.6 microm on the lateral axis and 
      1.0 microm on the apical side of the longitudinal axis; and (d) The dispersion of 
      the centromeres was not influenced by the degree of the nuclear decondensation. 
      We conclude that in human sperm nuclei, the acrocentric centromeres are organized 
      within a nonlocalized structural element in the chromocenter. The chromocenter 
      can range from an expanded size of 87% of the whole nucleus to a preferred size 
      of 8.3% independent of the degree of nuclear decondensation. These findings have 
      important implications for nuclear function (rRNA) that is not directly related 
      to sperm cell function or early embryo development.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Gurevitch, M
AU  - Gurevitch M
AD  - Male Fertility Laboratory, Faculty of Life Sciences, Bar-Ilan University, Ramat 
      Gan 52900, Israel.
FAU - Amiel, A
AU  - Amiel A
FAU - Ben-Zion, M
AU  - Ben-Zion M
FAU - Fejgin, M
AU  - Fejgin M
FAU - Bartoov, B
AU  - Bartoov B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (DNA Probes)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Cell Nucleus/genetics/*metabolism
MH  - Centromere/genetics/*metabolism
MH  - Chi-Square Distribution
MH  - DNA Probes/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - RNA, Ribosomal/metabolism
MH  - Spermatozoa/*cytology
EDAT- 2001/12/18 10:00
MHDA- 2002/02/13 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/02/13 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 10.1002/mrd.1116 [pii]
AID - 10.1002/mrd.1116 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2001 Dec;60(4):507-16. doi: 10.1002/mrd.1116.