PMID- 11746978
OWN - NLM
STAT- MEDLINE
DCOM- 20020110
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 32
IP  - 4
DP  - 2001 Dec
TI  - Interphase fluorescence in situ hybridization assay for the detection of 3q21 
      rearrangements in myeloid malignancies.
PG  - 373-80
AB  - In myeloid malignancies, chromosome rearrangements involving band 3q21 are 
      associated with a particularly poor prognosis of the disease. Their sensitive and 
      unequivocal detection is therefore of great clinical importance. In this report, 
      we describe the establishment of an interphase fluorescence in situ hybridization 
      (FISH) assay that complements classical cytogenetic analysis in the diagnosis of 
      such aberrations. PACs that map centromeric and telomeric of known 3q21 
      breakpoints were labeled with different fluorescent dyes, and the separation of 
      the normally colocalizing signals was used as an indicator of the presence of a 
      3q21 rearrangement. Two cell lines and 10 primary samples from myeloid leukemia 
      and myelodysplastic syndrome (MDS) patients with 3q21 rearrangements were 
      investigated using the newly established method. The rate of false positivity was 
      determined in 27 control samples from patients with various types of myeloid 
      malignancies. In addition to providing a sensitive and rapid test for the 
      detection of 3q21 aberrations, the interphase FISH assay yields preliminary 
      information about the localization of individual breakpoints. Six of the 10 
      breakpoints in the patient samples map to an only recently described breakpoint 
      cluster region (BCR) 60 kb centromeric of the originally reported 3q21 BCR. These 
      findings may contribute to the understanding of the molecular basis of the 
      clinical features associated with 3q21 rearrangements.
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Wieser, R
AU  - Wieser R
AD  - Institut fur Medizinische Biologie, Universitat Wien, Wien, Austria. 
      rotraud.wieser@univie.ac.at
FAU - Schreiner, U
AU  - Schreiner U
FAU - Pirc-Danoewinata, H
AU  - Pirc-Danoewinata H
FAU - Aytekin, M
AU  - Aytekin M
FAU - Schmidt, H H
AU  - Schmidt HH
FAU - Rieder, H
AU  - Rieder H
FAU - Fonatsch, C
AU  - Fonatsch C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Chromosome Breakage/genetics
MH  - *Chromosome Inversion
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - DNA Probes/genetics
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods
MH  - Interphase/*genetics
MH  - Leukemia, Myeloid/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics/pathology
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2001/12/18 10:00
MHDA- 2002/01/11 10:01
CRDT- 2001/12/18 10:00
PHST- 2001/12/18 10:00 [pubmed]
PHST- 2002/01/11 10:01 [medline]
PHST- 2001/12/18 10:00 [entrez]
AID - 10.1002/gcc.1202 [pii]
AID - 10.1002/gcc.1202 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2001 Dec;32(4):373-80. doi: 10.1002/gcc.1202.