PMID- 11747352
OWN - NLM
STAT- MEDLINE
DCOM- 20020208
LR  - 20201212
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 213
IP  - 1
DP  - 2001 Oct 10
TI  - Peripheral blood dendritic cells, but not monocyte-derived dendritic cells, can 
      augment human NK cell function.
PG  - 14-23
AB  - Dendritic cells (DCs) are essential antigen-presenting cells with a wide variety 
      of functions relating to both adaptive and innate immunity. Recently, 
      interactions of DCs with natural killer (NK) cells and NK1.1-positive T cells 
      have been reported in mice. However, in humans, this interaction is not well 
      understood. Here we report the use of a coculture method to analyze the 
      modulation of NK cell function in antitumor immunity by DCs. We found that 
      peripheral blood DCs (PDCs) enhanced NK cell activity in cytotoxicity assay, even 
      without direct contact between DC and NK cells. In contrast, neither 
      monocyte-derived DCs (MoDCs), nor TNF-alpha-treated MoDCs, stimulated NK lytic 
      activity. Secretion of IL-12 and TNF-alpha into the PDC-NK coculture supernatant 
      was increased. However, blocking antibodies against these cytokines could not 
      completely abolish the upregulation of NK activity, suggesting the presence of 
      other soluble factor(s) that affect DC-NK cell interaction. To summarize, this 
      study demonstrates for the first time the direct activation of human NK cells by 
      DC-NK cell interaction in vitro, suggesting that DCs may have a central role 
      linking the innate and adaptive immune responses. Moreover, in stimulating NK 
      cell function, PDCs appear to have a different potential from MoDCs.
CI  - (c)2001 Elsevier Science.
FAU - Osada, T
AU  - Osada T
AD  - Department of Transfusion Medicine, Graduate School of Medicine, University of 
      Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
FAU - Nagawa, H
AU  - Nagawa H
FAU - Kitayama, J
AU  - Kitayama J
FAU - Tsuno, N H
AU  - Tsuno NH
FAU - Ishihara, S
AU  - Ishihara S
FAU - Takamizawa, M
AU  - Takamizawa M
FAU - Shibata, Y
AU  - Shibata Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytotoxicity Tests, Immunologic
MH  - Dendritic Cells/cytology/*immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Interferon-gamma/metabolism
MH  - Interleukin-12/metabolism
MH  - Killer Cells, Natural/cytology/*immunology
MH  - Leukocytes, Mononuclear/cytology/immunology
MH  - Monocytes/cytology/immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2001/12/19 10:00
MHDA- 2002/02/09 10:01
CRDT- 2001/12/19 10:00
PHST- 2001/12/19 10:00 [pubmed]
PHST- 2002/02/09 10:01 [medline]
PHST- 2001/12/19 10:00 [entrez]
AID - S0008-8749(01)91858-1 [pii]
AID - 10.1006/cimm.2001.1858 [doi]
PST - ppublish
SO  - Cell Immunol. 2001 Oct 10;213(1):14-23. doi: 10.1006/cimm.2001.1858.