PMID- 11747993
OWN - NLM
STAT- MEDLINE
DCOM- 20020305
LR  - 20190921
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 14
IP  - 1
DP  - 2002 Jan
TI  - The P2X(7) receptor-mediated phospholipase D activation is regulated by both 
      PKC-dependent and PKC-independent pathways in a rat brain-derived Type-2 
      astrocyte cell line, RBA-2.
PG  - 83-92
AB  - The aim of this study was to characterize the regulatory mechanisms of the P2X(7) 
      receptor (P2X(7)R)-mediated phospholipase D (PLD) activation in a rat 
      brain-derived Type-2 astrocyte cell line, RBA-2. A time course study revealed 
      that activation of P2X(7)R resulted in a choline and not phosphorylcholine 
      formation, suggesting that activation of P2X(7)R is associated with the 
      phosphatidylcholine-PLD (PC-PLD) in these cells. GF 109203X, a selective protein 
      kinase C (PKC) inhibitor, partially inhibited the P2X(7)R-mediated PLD 
      activation, while blocking the phorbol 12-myristate 13-acetate (PMA)-stimulated 
      PLD activity. In addition, PMA synergistically activated the P2X(7)R-mediated PLD 
      activity. Furthermore, genistein, a tyrosine kinase inhibitor, blocked the 
      P2X(7)R-activated PLD, while KN62, a Ca(2+)/calmodulin-dependent protein kinase 
      II (CaMKII) inhibitor, was less effective, whereas the mitogen-activated protein 
      kinase (MAPK) inhibitor PD98059 was ineffective. No additive inhibitory effects 
      were found by simultaneous treatment of GF 109203X and KN62 on P2X(7)R-activated 
      PLD. Taken together, these results demonstrate that both PKC-dependent and 
      PKC-independent signaling pathways are involved in the regulation of 
      P2X(7)R-mediated PLD activation. Additionally, CaMKII may participate in the 
      PKC-dependent pathway, and tyrosine kinase may play a pivotal role on both 
      PKC-dependent and PKC-independent pathways in the P2X(7)R-mediated PLD activation 
      in RBA-2 cells.
FAU - Hung, Amos C
AU  - Hung AC
AD  - Institute of Neuroscience, College of Life Science, National Yang Ming 
      University, No. 155, Section 2, Li-Non Street, Shi-Pai, Taipei 112, Taiwan, ROC.
FAU - Sun, Synthia H
AU  - Sun SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indoles)
RN  - 0 (Maleimides)
RN  - 0 (P2rx7 protein, rat)
RN  - 0 (Purinergic P2 Receptor Agonists)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Purinergic P2)
RN  - 0 (Receptors, Purinergic P2X7)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (phosphatidylcholine-specific phospholipase C)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - L79H6N0V6C (bisindolylmaleimide I)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adenosine Triphosphate/pharmacology
MH  - Animals
MH  - Astrocytes/drug effects/enzymology/*metabolism
MH  - Brain/cytology
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/physiology
MH  - Cell Line
MH  - Drug Synergism
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Indoles/pharmacology
MH  - Kinetics
MH  - Maleimides/pharmacology
MH  - Phospholipase D/*metabolism
MH  - Protein Kinase C/antagonists & inhibitors/*physiology
MH  - Protein-Tyrosine Kinases/physiology
MH  - Purinergic P2 Receptor Agonists
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Receptors, Purinergic P2/genetics/*physiology
MH  - Receptors, Purinergic P2X7
MH  - *Signal Transduction
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Type C Phospholipases/metabolism
EDAT- 2001/12/19 10:00
MHDA- 2002/03/07 10:01
CRDT- 2001/12/19 10:00
PHST- 2001/12/19 10:00 [pubmed]
PHST- 2002/03/07 10:01 [medline]
PHST- 2001/12/19 10:00 [entrez]
AID - S0898656801002303 [pii]
AID - 10.1016/s0898-6568(01)00230-3 [doi]
PST - ppublish
SO  - Cell Signal. 2002 Jan;14(1):83-92. doi: 10.1016/s0898-6568(01)00230-3.