PMID- 11748297
OWN - NLM
STAT- MEDLINE
DCOM- 20020131
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 50
IP  - 1
DP  - 2002 Jan
TI  - Induction of p21 mRNA synthesis after short-wavelength UV light visualized in 
      individual cells by RNA FISH.
PG  - 81-9
AB  - Expression of the cyclin-dependent kinase inhibitor gene p21 is induced after DNA 
      damage and plays a role in cell survival. The exact mechanism of induction is not 
      known, but enhancement of mRNA stability has recently been implicated as an 
      important factor. To obtain further insight into the dynamics of p21 gene 
      expression at the individual cell level, normal fibroblasts, GM1492 fibroblasts 
      from a Bloom's syndrome patient, and U2OS osteosarcoma cells were UVC irradiated, 
      fixed at different time points, and subjected to mRNA fluorescence in situ 
      hybridization (FISH) and immunocytochemical staining. In mock-irradiated normal 
      fibroblasts, a subfraction of cells revealed low levels of p21 mRNA synthesis. 
      After UVC treatment, p21 transcripts accumulated over time in nuclear locations 
      other than transcription foci. At 6 hr after irradiation, almost 50% of the cells 
      displayed p21 mRNA in three different distribution patterns within the nuclei. 
      The highest frequency of cells with cytoplasmic accumulation of p21 mRNA was seen 
      at 17 hr after UVC treatment. We conclude that increased p21 gene transcription 
      and possibly stabilization of newly synthesized p21 mRNA contribute to elevated 
      levels of p21 protein after UVC irradiation. (J Histochem Cytochem 50:81-89, 
      2002)
FAU - Hattinger, Claudia M
AU  - Hattinger CM
AD  - Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, 
      The Netherlands.
FAU - Jochemsen, Aart G
AU  - Jochemsen AG
FAU - Tanke, Hans J
AU  - Tanke HJ
FAU - Dirks, Roeland W
AU  - Dirks RW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclins/biosynthesis/*metabolism
MH  - Cytoplasm/metabolism
MH  - Fibroblasts/metabolism/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Microscopy, Fluorescence
MH  - RNA, Messenger/biosynthesis/*metabolism
MH  - Tumor Cells, Cultured
MH  - *Ultraviolet Rays
EDAT- 2001/12/19 10:00
MHDA- 2002/02/01 10:01
CRDT- 2001/12/19 10:00
PHST- 2001/12/19 10:00 [pubmed]
PHST- 2002/02/01 10:01 [medline]
PHST- 2001/12/19 10:00 [entrez]
AID - 10.1177/002215540205000109 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2002 Jan;50(1):81-9. doi: 10.1177/002215540205000109.