PMID- 11748657
OWN - NLM
STAT- MEDLINE
DCOM- 20020205
LR  - 20190901
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 66
IP  - 1
DP  - 2002 Jan
TI  - Occurrence of hepatitis E virus IgM, low avidity IgG serum antibodies, and 
      viremia in sporadic cases of non-A, -B, and -C acute hepatitis.
PG  - 40-8
AB  - Serum samples were taken from 57 patients with sporadic non-A, -B, and -C (Non A, 
      B, C) acute hepatitis at different times after onset of the disease and tested 
      for the presence of the hepatitis E virus (HEV) RNA, IgM, and low avidity IgG 
      antibodies. The viral antibodies were detected using two ELISA. One assay (GL) 
      was produced using a mixture of recombinant peptides specified by ORF2 and ORF3 
      of the viral genome. The other was produced with an ORF2 specified peptide, pE2. 
      The latter occurs naturally as homodimer, it is recognized strongly in its 
      dimeric form by human sera and, in the primate model, it confers protection 
      against experimental HEV infection. Nineteen samples were positive for one or 
      more of these acute markers of HEV infection, 14 of which were acute sera with 
      elevated ALT levels and 5 were convalescent sera with normal ALT level. The 
      results showed that icteric phase of sporadic hepatitis lasts for about 17 days 
      and it coincides with a period when viremia is subsiding as HEV antibodies are 
      developing. Viremia was intermittent and all but one of the 5 instances were 
      confined to the icteric phase with elevated ALT levels. On two of these 
      occasions, viremia preceded detection of HEV antibody, on another 2 occasions it 
      was concurrent with the detection of pE2 specific IgM and/or low avidity IgG and 
      only in one case of protracted viremia was the viral genome detected concurrently 
      with avid pE2 IgG antibody. Ten (71%) of the 14 acute sera were reactive for pE2 
      IgM, eight (57%) were reactive for low avidity pE2 IgG, and six (43%) for the GL 
      IgM. The sensitivity for the diagnosis of acute hepatitis E may be increased to 
      87% by combining pE2 IgM and viremia. GL IgM was detected later, but persisted 
      for a longer period of time than the pE2 antibodies, and it was the only acute 
      antibody detected in the convalescent sera.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Zhang, J Z
AU  - Zhang JZ
AD  - Department of Microbiology, Faculty of Medicine, The University of Hong Kong, 
      Hong Kong SAR, P.R. China.
FAU - Im, Stanley W K
AU  - Im SW
FAU - Lau, S H
AU  - Lau SH
FAU - Chau, T N
AU  - Chau TN
FAU - Lai, S T
AU  - Lai ST
FAU - Ng, S P
AU  - Ng SP
FAU - Peiris, Malik
AU  - Peiris M
FAU - Tse, Cindy
AU  - Tse C
FAU - Ng, T K
AU  - Ng TK
FAU - Ng, M H
AU  - Ng MH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Hepatitis Antibodies)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Acute Disease
MH  - *Antibody Affinity
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Hepatitis Antibodies/*blood
MH  - Hepatitis E/*diagnosis/immunology/virology
MH  - Hepatitis E virus/*immunology/isolation & purification
MH  - Humans
MH  - Immunoglobulin G/*blood
MH  - Immunoglobulin M/*blood
MH  - RNA, Viral/blood
MH  - Viremia/virology
EDAT- 2001/12/19 10:00
MHDA- 2002/02/06 10:01
CRDT- 2001/12/19 10:00
PHST- 2001/12/19 10:00 [pubmed]
PHST- 2002/02/06 10:01 [medline]
PHST- 2001/12/19 10:00 [entrez]
AID - 10.1002/jmv.2109 [pii]
AID - 10.1002/jmv.2109 [doi]
PST - ppublish
SO  - J Med Virol. 2002 Jan;66(1):40-8. doi: 10.1002/jmv.2109.