PMID- 11748686
OWN - NLM
STAT- MEDLINE
DCOM- 20020211
LR  - 20131121
IS  - 0269-3879 (Print)
IS  - 0269-3879 (Linking)
VI  - 15
IP  - 8
DP  - 2001 Dec
TI  - RP-HPLC method with electrochemical detection for the determination of 
      metoclopramide in serum and its use in pharmacokinetic studies.
PG  - 513-7
AB  - A rapid and sensitive reversed-phase high performance liquid chromatographic 
      method has been developed for the determination of metoclopramide in serum. The 
      assay was performed after single extraction with ethyl ether using methyl 
      parahydroxybenzoate as internal standard. Chromatographic separations were 
      performed on C(18) stationary phase with a mobile phase composed of 
      methanol-phosphate buffer pH 3 (30:70 v/v). Analytes were detected 
      electrochemically. The quantification limit for metoclopramide in serum was 2 ng 
      mL(-1). Linearity of the method was confirmed in the range of 5-120 ng mL(-1) 
      (correlation coefficient 0.9998). Within-day relative standard deviations (RSDs) 
      ranged from 0.3 to 5.5% and between-day RSDs from 0.8 to 6.0%. The analytical 
      method was successfully applied for the determination of pharmacokinetic 
      parameters after ingestion of 10 mg dose of metoclopramide. Studies were 
      performed on 18 healthy volunteers of both sexes.
CI  - Copyright 2001 John Wiley & Sons, Ltd.
FAU - Lamparczyk, H
AU  - Lamparczyk H
AD  - Medical University of Gdansk, Faculty of Pharmacy, Hallera 107, PL-80-416 Gdansk, 
      Poland. lamparcz@eniac.farmacja.amg.gda.pl
FAU - Chmielewska, A
AU  - Chmielewska A
FAU - Konieczna, L
AU  - Konieczna L
FAU - Plenis, A
AU  - Plenis A
FAU - Zarzycki, P K
AU  - Zarzycki PK
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Antiemetics)
RN  - 0 (Dopamine Antagonists)
RN  - L4YEB44I46 (Metoclopramide)
SB  - IM
MH  - Administration, Oral
MH  - Adult
MH  - Antiemetics/blood
MH  - Area Under Curve
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Dopamine Antagonists/blood
MH  - Electrochemistry
MH  - Female
MH  - Humans
MH  - Male
MH  - Metoclopramide/*blood/pharmacokinetics
MH  - Reproducibility of Results
EDAT- 2001/12/19 10:00
MHDA- 2002/02/12 10:01
CRDT- 2001/12/19 10:00
PHST- 2001/12/19 10:00 [pubmed]
PHST- 2002/02/12 10:01 [medline]
PHST- 2001/12/19 10:00 [entrez]
AID - 10.1002/bmc.101 [pii]
AID - 10.1002/bmc.101 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2001 Dec;15(8):513-7. doi: 10.1002/bmc.101.