PMID- 11749848
OWN - NLM
STAT- MEDLINE
DCOM- 20040112
LR  - 20061115
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 22
IP  - 8
DP  - 2001 Aug
TI  - Roles of IL-4 and other factors in the trichosanthin-induced ovalbumin-specific 
      IgE response.
PG  - 736-40
AB  - AIM: To study the mechanism of trichosanthin (TCS)-induced ovalbumin 
      (OVA)-specific immunoglobulin E (IgE) response in vivo. METHODS: To determine 
      whether interleukin-4 (IL-4) was involved in TCS-induced IgE production, TCS and 
      OVA co-immunized mice were treated with anti-IL-4 monoclonal antibodies (mAb) and 
      OVA-specific serum IgE production was measured by enzyme-linked immunosorbent 
      assay (ELISA). To distinguish whether recombinant IL-4 (rIL-4) was sufficient to 
      support OVA-specific IgE response, OVA alone immunized mice were treated with 
      rIL-4 and OVA-induced IgE production were examined by ELISA in the serum. To 
      determine whether additional factors were involved in TCS-induced IgE response, 
      th e kinetic expression of CD40 ligand (CD40L), tumor necrosis factor-alpha 
      (TNF-alpha), and interleukin-13 (IL-13) were measured by semi-quantitative RT-PCR 
      in draining mesenteric lymph node (MLN) from TCS-immunized mice. RESULTS: 
      TCS-induced OVA-specific IgE production was suppressed by anti-IL-4 antibody, 
      whereas IL-4 alone could not induce OVA-specific IgE production. CD40L, 
      TNF-alpha, and IL-13 all expressed high levels in MLN after both primary and 
      secondary immune responses. Among them CD40L had the similar transient expression 
      peak to that of IL-4. CONCLUSION: IL-4 was indispensable for TCS-induced 
      OVA-specific IgE production, and the other three factors examined may also be 
      involved in, but CD40L may play a more important role.
FAU - Xu, W F
AU  - Xu WF
AD  - Laboratory of Molecular Immunology, Institute of Biochemistry and Cell Biology, 
      Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 
      Shanghai 200031, China.
FAU - Ji, Y Y
AU  - Ji YY
FAU - Wu, Y D
AU  - Wu YD
FAU - Lin, G M
AU  - Lin GM
FAU - Ye, M
AU  - Ye M
FAU - Sun, B
AU  - Sun B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Interleukin-13)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 147205-72-9 (CD40 Ligand)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 60318-52-7 (Trichosanthin)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - CD40 Ligand/pharmacology
MH  - Female
MH  - Immunoglobulin E/biosynthesis/*immunology
MH  - Interleukin-13/pharmacology
MH  - Interleukin-4/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Ovalbumin/*immunology
MH  - Trichosanthin/*pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2001/12/26 10:00
MHDA- 2004/01/13 05:00
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2004/01/13 05:00 [medline]
PHST- 2001/12/26 10:00 [entrez]
PST - ppublish
SO  - Acta Pharmacol Sin. 2001 Aug;22(8):736-40.