PMID- 11749970
OWN - NLM
STAT- MEDLINE
DCOM- 20020125
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 509
IP  - 3
DP  - 2001 Dec 14
TI  - Human macrophage inflammatory protein-3alpha/CCL20/LARC/Exodus/SCYA20 is 
      transcriptionally upregulated by tumor necrosis factor-alpha via a non-standard 
      NF-kappaB site.
PG  - 439-45
AB  - The 5'-flanking sequences of the human macrophage inflammatory 
      protein-3alpha/CCL20 gene were cloned and transfected into G-361 human melanoma 
      cells in a luciferase reporter construct. Tumor necrosis factor-alpha (TNF-alpha) 
      treatment stimulated luciferase expression, and promoter truncations demonstrated 
      that TNF-alpha inducibility is conferred by a region between nt -111 and -77, 
      which contains a non-standard nuclear factor-kappaB (NF-kappaB) binding site. The 
      requirement for NF-kappaB was demonstrated as follows: (i) mutations in this 
      NF-kappaB site abrogated TNF-alpha responsiveness; (ii) TNF-alpha activated a 
      construct containing two copies of the CCL20 NF-kappaB binding site; (iii) 
      overexpression of NF-kappaB p65 activated the CCL20 promoter; (iv) NF-kappaB from 
      nuclear extracts of TNF-alpha-stimulated cells bound specifically to this 
      NF-kappaB site.
FAU - Harant, H
AU  - Harant H
AD  - Department of Inflammatory Diseases, Novartis Research Institute, Vienna, 
      Austria.
FAU - Eldershaw, S A
AU  - Eldershaw SA
FAU - Lindley, I J
AU  - Lindley IJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (CCL20 protein, human)
RN  - 0 (CCR6 protein, human)
RN  - 0 (Chemokine CCL20)
RN  - 0 (Chemokines, CC)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, CCR6)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - 5' Flanking Region/genetics
MH  - Base Sequence
MH  - Cell Line
MH  - Chemokine CCL20
MH  - Chemokines, CC/*genetics
MH  - Cloning, Molecular
MH  - Electrophoretic Mobility Shift Assay
MH  - Humans
MH  - Luciferases/genetics/metabolism
MH  - Macrophage Inflammatory Proteins/*genetics
MH  - Molecular Sequence Data
MH  - NF-kappa B/*metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding
MH  - Receptors, CCR6
MH  - *Receptors, Chemokine
MH  - Response Elements/*genetics
MH  - Transcription Factor RelA
MH  - Transcription, Genetic/*drug effects
MH  - Transcriptional Activation/*drug effects
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2001/12/26 10:00
MHDA- 2002/01/26 10:01
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/01/26 10:01 [medline]
PHST- 2001/12/26 10:00 [entrez]
AID - S0014-5793(01)03138-6 [pii]
AID - 10.1016/s0014-5793(01)03138-6 [doi]
PST - ppublish
SO  - FEBS Lett. 2001 Dec 14;509(3):439-45. doi: 10.1016/s0014-5793(01)03138-6.