PMID- 11750043
OWN - NLM
STAT- MEDLINE
DCOM- 20020605
LR  - 20190826
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 80
IP  - 2
DP  - 2002 Feb 1
TI  - Effect of dialkyl phthalates on the degranulation and Ca2+ response of RBL-2H3 
      mast cells.
PG  - 119-24
AB  - We examined the effect of three dialkyl phthalates, di-n-butylphthalate (DBP), 
      di-isobutylphthalate (DIBP) and di(2-ethylhexyl)phthalate (DEHP), on 
      antigen-induced degranulation of RBL-2H3 mast cells. Exposure to 50-500 microM 
      DBP, 50-500 microM DIBP, and 500 microM DEHP significantly potentiated 
      antigen-induced beta-hexosaminidase release. Without antigen stimulation, the 
      phthalates did not cause any significant increase in degranulation. Next, we 
      examined the Ca2+ response of RBL-2H3 cells after exposure to these phthalates. 
      The cytosolic calcium ion concentration ([Ca2+]i) of the cells clearly increased 
      when the cells were stimulated with 50-500 microM and 50-500 microM DIBP, and 
      increased slightly when stimulated with 50-500 microM DEHP. Digital imaging 
      fluorescence microscope analysis showed that the addition of DBP evoked Ca2+ 
      oscillation in individual mast cells. Finally, we investigated the relationship 
      between the DBP-sensitive Ca2+ stores and thapsigargin (TG)-sensitive Ca2+ 
      stores. A rise in [Ca2+](i) following challenge with DBP after TG was observed, 
      and thus the DBP-sensitive and TG-sensitive Ca2+ stores in RBL-2H3 cells seem to 
      be different. In conclusion, some dialkyl phthalates increase antigen-induced 
      degranulation in RBL-2H3 cells dependent on the increase of [Ca2+]i.
FAU - Nakamura, Ryosuke
AU  - Nakamura R
AD  - Division of Biochemistry and Immunochemistry, National Institute of Health 
      Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158-8501, Japan.
FAU - Teshima, Reiko
AU  - Teshima R
FAU - Sawada, Jun ichi
AU  - Sawada Ji
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 2286E5R2KE (Dibutyl Phthalate)
RN  - 56092-81-0 (Ionomycin)
RN  - C42K0PH13C (Diethylhexyl Phthalate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Calcium Signaling/*drug effects
MH  - Cell Degranulation/*drug effects
MH  - Dibutyl Phthalate/analogs & derivatives/*pharmacology
MH  - Diethylhexyl Phthalate/*pharmacology
MH  - Ionomycin/metabolism
MH  - Mast Cells/cytology/*drug effects/metabolism
MH  - Rats
MH  - Time Factors
MH  - Tumor Cells, Cultured
EDAT- 2001/12/26 10:00
MHDA- 2002/06/06 10:01
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/06/06 10:01 [medline]
PHST- 2001/12/26 10:00 [entrez]
AID - S0165247801003182 [pii]
AID - 10.1016/s0165-2478(01)00318-2 [doi]
PST - ppublish
SO  - Immunol Lett. 2002 Feb 1;80(2):119-24. doi: 10.1016/s0165-2478(01)00318-2.