PMID- 11751168
OWN - NLM
STAT- MEDLINE
DCOM- 20020131
LR  - 20141120
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 282
IP  - 1
DP  - 2002 Jan
TI  - MCP-1 causes leukocyte recruitment and subsequently endotoxemic ileus in rat.
PG  - G145-55
AB  - Endotoxemia causes an inflammatory response within the intestinal muscularis and 
      gastrointestinal dysmotility. We hypothesize that the resident macrophage-derived 
      chemokine monocyte chemoattractant protein-1 (MCP-1) plays a significant role in 
      the recruitment of leukocytes into the lipopolysaccharide (LPS)-stimulated rat 
      intestinal muscularis. MCP-1 mRNA expression was investigated by RT-PCR. 
      Leukocyte extravasation and MCP-1 protein localization were determined by 
      immunohistochemistry. Contractile activity was assessed by using a standard organ 
      bath in rats that were treated with saline, recombinant MCP-1, LPS, LPS + 
      nonspecific antibody, or LPS + MCP-1 antibody. Endotoxemia caused a significant 
      280-fold increase in MCP-1 mRNA expression in the muscularis, peaking at 3 h. 
      MCP-1 protein was immunohistochemically located to muscularis macrophages. LPS 
      application caused significant leukocyte recruitment into the muscularis and a 
      51% decrease in muscle contractility. MCP-1 antibody treatment significantly 
      averted leukocyte recruitment and significantly prevented muscle dysfunction. 
      These parameters were not significantly altered by the nonspecific antibody. 
      Results show that resident muscularis macrophage-derived MCP-1 plays a major role 
      in the recruitment of monocytes during endotoxemia, which then subsequently 
      secrete kinetically active substances that cause ileus.
FAU - Turler, Andreas
AU  - Turler A
AD  - Department of Medicine, Division of Gastroenterology, University of Pittsburgh 
      Medical Center, Pittsburgh, Pennsylvania 15261, USA.
FAU - Schwarz, Nicolas T
AU  - Schwarz NT
FAU - Turler, Esther
AU  - Turler E
FAU - Kalff, Jorg C
AU  - Kalff JC
FAU - Bauer, Anthony J
AU  - Bauer AJ
LA  - eng
GR  - P50-GM-53789/GM/NIGMS NIH HHS/United States
GR  - R01-GM-58241/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (Antibodies)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Chemokine CCL2/*genetics/immunology/*pharmacology
MH  - Chemotaxis, Leukocyte/*drug effects
MH  - Endotoxemia/*immunology/physiopathology
MH  - Gastrointestinal Motility/immunology
MH  - Gene Expression/drug effects/immunology
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Intestinal Obstruction/*immunology/physiopathology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/physiology
MH  - Male
MH  - Muscle, Smooth/immunology/physiopathology
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Inbred ACI
EDAT- 2001/12/26 10:00
MHDA- 2002/02/01 10:01
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/02/01 10:01 [medline]
PHST- 2001/12/26 10:00 [entrez]
AID - 10.1152/ajpgi.00263.2001 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2002 Jan;282(1):G145-55. doi: 
      10.1152/ajpgi.00263.2001.