PMID- 11751623
OWN - NLM
STAT- MEDLINE
DCOM- 20020117
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 143
IP  - 1
DP  - 2002 Jan
TI  - Evaluation of the effects of 17beta-estradiol (17beta-e2) on gene expression in 
      experimental autoimmune encephalomyelitis using DNA microarray.
PG  - 313-9
AB  - The aim of this study was to identify immune-related genes affected by treatment 
      with 17beta-estradiol (17beta-E2) that contribute to protection of T cell antigen 
      receptor double transgenic mice from experimental autoimmune encephalomyelitis 
      (EAE). The Affymetrix microarray system was used to screen more than 12,000 genes 
      from E2-treated mice protected from EAE vs. control mice with severe EAE. In 
      general, E2 treatment affected about 10% of the genes tested, but only 18 
      cytokine, chemokine/receptor, adhesion molecule, or activation genes were up- or 
      down-regulated more than 2.4-fold by E2 treatment. Down-regulated genes included 
      TNFalpha (an important proinflammatory cytokine in EAE); peptidoglycan 
      recognition proteins (Pgrp); regulated on activation, normal T cell expressed and 
      secreted (RANTES); and neural cell adhesion molecule (MCP-1). Up-regulated genes 
      included cytotoxic T lymphocyte antigen-4 (CTLA-4; known to inhibit T cell 
      activation), TGFbeta3, IL-18, and two interferon-gamma-induced genes, the 
      chemokines: monocyte chemoattractant protein-1 (MCP-1) and macrophage 
      inflammatory protein-1beta (MIP-1beta), vascular cell adhesion molecule (VCAM), 
      and disintegrin metalloprotease (thought to regulate TNFalpha production). These 
      results implicate a limited set of known and previously unsuspected E2-sensitive 
      genes that may be crucial for inhibition of EAE and potentially the human 
      disease, multiple sclerosis.
FAU - Matejuk, Agata
AU  - Matejuk A
AD  - Department of Neurology, Oregon Health Sciences University, Portland, OR 97201, 
      USA.
FAU - Dwyer, Jami
AU  - Dwyer J
FAU - Zamora, Alex
AU  - Zamora A
FAU - Vandenbark, Arthur A
AU  - Vandenbark AA
FAU - Offner, Halina
AU  - Offner H
LA  - eng
GR  - AI-42376/AI/NIAID NIH HHS/United States
GR  - NS-23221/NS/NINDS NIH HHS/United States
GR  - NS-23444/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 4TI98Z838E (Estradiol)
RN  - 9007-81-2 (Freund's Adjuvant)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Encephalomyelitis, Autoimmune, Experimental/*genetics
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Freund's Adjuvant/pharmacology
MH  - Gene Expression/*drug effects
MH  - Mice
MH  - Mice, Transgenic
MH  - Nuclease Protection Assays
MH  - Oligonucleotide Array Sequence Analysis
MH  - Ribonucleases
EDAT- 2001/12/26 10:00
MHDA- 2002/01/18 10:01
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/01/18 10:01 [medline]
PHST- 2001/12/26 10:00 [entrez]
AID - 10.1210/endo.143.1.8571 [doi]
PST - ppublish
SO  - Endocrinology. 2002 Jan;143(1):313-9. doi: 10.1210/endo.143.1.8571.