PMID- 11752175
OWN - NLM
STAT- MEDLINE
DCOM- 20020111
LR  - 20231213
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 76
IP  - 2
DP  - 2002 Jan
TI  - Intracellular localization of the peanut clump virus replication complex in 
      tobacco BY-2 protoplasts containing green fluorescent protein-labeled endoplasmic 
      reticulum or Golgi apparatus.
PG  - 865-74
AB  - RNA-1 of Peanut clump virus (PCV) encodes the proteins P131 and P191, containing 
      the signature motifs of replication proteins, and P15, which regulates viral RNA 
      accumulation. In PCV-infected protoplasts both P131 and P191 were immunodetected 
      in the perinuclear region. Laser scanning confocal microscopy (LSCM) showed that 
      P131 and P191 colocalized with neosynthesized 5-bromouridine 
      5'-triphosphate-labeled RNA and double-stranded RNA, demonstrating that they 
      belong to the replication complex. On the contrary, the P15 fused to the enhanced 
      green fluorescent protein (EGFP) never colocalized with the two proteins. In 
      endoplasmic reticulum (ER)-GFP transgenic BY-2 protoplasts, the distribution of 
      the green fluorescent-labeled ER was strongly modified by PCV infection. LSCM 
      showed that both P131 and P191 colocalized with ER green fluorescent bodies 
      accumulating around the nucleus during infection. The replication process was not 
      inhibited by cerulenin and brefeldin A, suggesting that PCV replication does not 
      depend on de novo-synthesized membrane and does not require transport through the 
      Golgi apparatus. Electron microscopy of ultrathin sections of infected 
      protoplasts showed aggregates of broken ER but also visualized vesicles, some of 
      which resembled modified peroxisomes. The results suggest that accumulation of 
      PCV during infection is accompanied by specific association of PCV RNA-1-encoded 
      proteins with membranes of the ER and other organelles. The concomitant extensive 
      rearrangement of these membranous structures leads to the formation of 
      intracellular compartments in which synthesis and accumulation of the viral RNA 
      occur in defined areas.
FAU - Dunoyer, Patrice
AU  - Dunoyer P
AD  - Institut de Biologie Moleculaire des Plantes, Centre National de la Recherche 
      Scientifique, 67084 Strasbourg Cedex, France.
FAU - Ritzenthaler, Christophe
AU  - Ritzenthaler C
FAU - Hemmer, Odile
AU  - Hemmer O
FAU - Michler, Pierre
AU  - Michler P
FAU - Fritsch, Christiane
AU  - Fritsch C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Viral)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Proteins)
RN  - 17397-89-6 (Cerulenin)
RN  - 20350-15-6 (Brefeldin A)
SB  - IM
MH  - Arachis/*virology
MH  - Brefeldin A/pharmacology
MH  - Cerulenin/pharmacology
MH  - Endoplasmic Reticulum/pathology/ultrastructure/*virology
MH  - Golgi Apparatus/pathology/ultrastructure/*virology
MH  - Intracellular Membranes/drug effects/virology
MH  - Microscopy, Electron
MH  - Molecular Weight
MH  - Plant Viruses/chemistry/drug effects/*physiology/ultrastructure
MH  - Plants, Genetically Modified
MH  - Protein Transport/drug effects
MH  - Protoplasts/ultrastructure/*virology
MH  - RNA, Viral/analysis/biosynthesis/genetics
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Nicotiana/ultrastructure/*virology
MH  - Viral Proteins/*analysis/biosynthesis/chemistry/metabolism
MH  - *Virus Replication/drug effects
PMC - PMC136813
EDAT- 2001/12/26 10:00
MHDA- 2002/01/12 10:01
PMCR- 2002/01/01
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/01/12 10:01 [medline]
PHST- 2001/12/26 10:00 [entrez]
PHST- 2002/01/01 00:00 [pmc-release]
AID - 1140 [pii]
AID - 10.1128/jvi.76.2.865-874.2002 [doi]
PST - ppublish
SO  - J Virol. 2002 Jan;76(2):865-74. doi: 10.1128/jvi.76.2.865-874.2002.