PMID- 11752240
OWN - NLM
STAT- MEDLINE
DCOM- 20020520
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 17
IP  - 1
DP  - 2002 Jan
TI  - Detection of micronuclei, cell proliferation and hyperdiploidy in bladder 
      epithelial cells of rats treated with o-phenylphenol.
PG  - 89-93
AB  - o-Phenylphenol (OPP), a widely used fungicide and antibacterial agent, has been 
      considered to be among the top 10 home and garden pesticides used in the USA. 
      Earlier studies have consistently shown that the sodium salt of OPP (SOPP) causes 
      bladder cancer in male Fischer 344 (F344) rats, whereas OPP has produced variable 
      results. This difference has been attributed to the presence of the sodium salt. 
      To determine cellular and genetic alterations in the rat bladder and the 
      influence of the sodium salt, F344 rats were administered 2% OPP, 2% NaCl and 2% 
      NaCl + 2% OPP in their diet for 14 days. Twenty-four hours before being killed 
      the animals were administered 5-bromo-2'-deoxyuridine (BrdU) by i.p. injection. 
      Bladder cells were isolated, stained with DAPI and scored for the presence of 
      micronuclei and incorporation of BrdU into replicating cells. To determine 
      changes in chromosome number, we used fluorescence in situ hybridization (FISH) 
      with a DNA probe for rat chromosome 4. Significant increases in the frequency of 
      micronuclei and BrdU incorporation were seen in bladder cells of rats from all 
      treatment groups. In contrast, the frequency of hyperdiploidy/polyploidy in 
      treated animals was not increased over that seen in controls. A high control 
      frequency of cells with three or more hybridization signals was seen, probably 
      due to the presence of polyploid cells in the bladder. The presence of polyploid 
      cells combined with cytotoxicity and compensatory cell proliferation makes it 
      difficult to determine whether OPP is capable of inducing aneuploidy in the rat 
      urothelium. In summary, these studies show that OPP can cause cellular and 
      chromosomal alterations in rat bladder cells in the absence of the sodium salt. 
      These results also indicate that at high concentrations the sodium salt can 
      enhance chromosomal damage in the rat urothelium.
FAU - Balakrishnan, S
AU  - Balakrishnan S
AD  - Environmental Toxicology Graduate Program, University of California, Riverside, 
      CA 92521, USA.
FAU - Uppala, P T
AU  - Uppala PT
FAU - Rupa, D S
AU  - Rupa DS
FAU - Hasegawa, L
AU  - Hasegawa L
FAU - Eastmond, D A
AU  - Eastmond DA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Fungicides, Industrial)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - D343Z75HT8 (2-phenylphenol)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Aneuploidy
MH  - Animals
MH  - Biphenyl Compounds/*toxicity
MH  - Body Weight/drug effects
MH  - Bromodeoxyuridine/analysis
MH  - Cell Division/drug effects
MH  - Chromosomes/drug effects/ultrastructure
MH  - DNA Replication/drug effects
MH  - Drug Synergism
MH  - Endpoint Determination
MH  - Epithelial Cells/drug effects/ultrastructure
MH  - Fungicides, Industrial/*toxicity
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Micronucleus Tests
MH  - *Polyploidy
MH  - Rats
MH  - Rats, Inbred F344
MH  - Sodium Chloride/pharmacology
MH  - Urinary Bladder/cytology/*drug effects
MH  - Urothelium/cytology/drug effects
EDAT- 2001/12/26 10:00
MHDA- 2002/05/22 10:01
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/05/22 10:01 [medline]
PHST- 2001/12/26 10:00 [entrez]
AID - 10.1093/mutage/17.1.89 [doi]
PST - ppublish
SO  - Mutagenesis. 2002 Jan;17(1):89-93. doi: 10.1093/mutage/17.1.89.