PMID- 11752392
OWN - NLM
STAT- MEDLINE
DCOM- 20020415
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 99
IP  - 1
DP  - 2002 Jan 8
TI  - Photic and circadian expression of luciferase in mPeriod1-luc transgenic mice 
      invivo.
PG  - 489-94
AB  - A conserved transcription-translation negative feedback loop forms the molecular 
      basis of the circadian oscillator in animals. Molecular interactions within this 
      loop have been relatively well characterized in vitro and in cell culture; 
      however, in vivo approaches are required to assess the functional significance of 
      these interactions. Here, regulation of circadian gene expression was studied in 
      vivo by using transgenic reporter mouse lines in which 6.75 kb of the mouse 
      Period1 (mPer1) promoter drives luciferase (luc) expression. Six mPer1-luc 
      transgenic lines were created, and all lines express a daily rhythm of luc mRNA 
      in the suprachiasmatic nuclei (SCN). Each mPer1-luc line also sustains a 
      long-term circadian rhythm of luminescence in SCN slice culture. A 6-h light 
      pulse administered during the early subjective night rapidly induces luc mRNA 
      expression in the SCN; however, high luc mRNA levels are sustained, whereas 
      endogenous mPer1 mRNA levels return to baseline, suggesting that 
      posttranscriptional events mediate the down-regulation of mPer1 after exposure to 
      light. This approach demonstrates that the 6.75-kb mPer1 promoter fragment is 
      sufficient to confer both circadian and photic regulation in vivo and reveals a 
      potential posttranscriptional regulatory mechanism within the mammalian circadian 
      oscillator.
FAU - Wilsbacher, Lisa D
AU  - Wilsbacher LD
AD  - Howard Hughes Medical Institute, 2153 North Campus Drive, Northwestern 
      University, Evanston, IL 60208, USA.
FAU - Yamazaki, Shin
AU  - Yamazaki S
FAU - Herzog, Erik D
AU  - Herzog ED
FAU - Song, Eun-Joo
AU  - Song EJ
FAU - Radcliffe, Laurel A
AU  - Radcliffe LA
FAU - Abe, Michikazu
AU  - Abe M
FAU - Block, Gene
AU  - Block G
FAU - Spitznagel, Edward
AU  - Spitznagel E
FAU - Menaker, Michael
AU  - Menaker M
FAU - Takahashi, Joseph S
AU  - Takahashi JS
LA  - eng
GR  - R37 MH39592/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20011218
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Per1 protein, mouse)
RN  - 0 (Period Circadian Proteins)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Cycle Proteins
MH  - Cells, Cultured
MH  - *Circadian Rhythm
MH  - DNA/metabolism
MH  - Genes, Reporter
MH  - In Situ Hybridization
MH  - *Light
MH  - Luciferases/*biosynthesis/*genetics/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Models, Theoretical
MH  - Nuclear Proteins/*biosynthesis/*genetics
MH  - Period Circadian Proteins
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Suprachiasmatic Nucleus/metabolism
MH  - Time Factors
MH  - Transfection
PMC - PMC117587
EDAT- 2001/12/26 10:00
MHDA- 2002/04/16 10:01
PMCR- 2002/07/08
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/04/16 10:01 [medline]
PHST- 2001/12/26 10:00 [entrez]
PHST- 2002/07/08 00:00 [pmc-release]
AID - 012248599 [pii]
AID - 2485 [pii]
AID - 10.1073/pnas.012248599 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):489-94. doi: 10.1073/pnas.012248599. 
      Epub 2001 Dec 18.