PMID- 11752707 OWN - NLM STAT- MEDLINE DCOM- 20020219 LR - 20200219 IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 83 IP - Pt 1 DP - 2002 Jan TI - Diversity of mosaic structures and common ancestry of human immunodeficiency virus type 1 BF intersubtype recombinant viruses from Argentina revealed by analysis of near full-length genome sequences. PG - 107-119 LID - 10.1099/0022-1317-83-1-107 [doi] AB - The findings that BF intersubtype recombinant human immunodeficiency type 1 viruses (HIV-1) with coincident breakpoints in pol are circulating widely in Argentina and that non-recombinant F subtype viruses have failed to be detected in this country were reported recently. To analyse the mosaic structures of these viruses and to determine their phylogenetic relationship, near full-length proviral genomes of eight of these recombinant viruses were amplified by PCR and sequenced. Intersubtype breakpoints were analysed by bootscanning and examining the signature nucleotides. Phylogenetic relationships were determined with neighbour-joining trees. Five viruses, each with predominantly subtype F genomes, exhibited mosaic structures that were highly similar. Two intersubtype breakpoints were shared by all viruses and seven by the majority. Of the consensus breakpoints, all nine were present in two viruses, which exhibited identical recombinant structures, and four to eight breakpoints were present in the remaining viruses. Phylogenetic analysis of partial sequences supported both a common ancestry, at least in part of their genomes, for all recombinant viruses and the phylogenetic relationship of F subtype segments with F subtype viruses from Brazil. A common ancestry of the recombinants was supported also by the presence of shared signature amino acids and nucleotides, either unreported or highly unusual in F and B subtype viruses. These results indicate that HIV-1 BF recombinant viruses with diverse mosaic structures, including a circulating recombinant form (which are widespread in Argentina) derive from a common recombinant ancestor and that F subtype segments of these recombinants are related phylogenetically to the F subtype viruses from Brazil. FAU - Thomson, Michael M AU - Thomson MM AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Delgado, Elena AU - Delgado E AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Herrero, Isabel AU - Herrero I AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Villahermosa, Maria Luisa AU - Villahermosa ML AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Vazquez-de Parga, Elena AU - Vazquez-de Parga E AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Cuevas, Maria Teresa AU - Cuevas MT AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Carmona, Rocio AU - Carmona R AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Medrano, Leandro AU - Medrano L AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Perez-Alvarez, Lucia AU - Perez-Alvarez L AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Cuevas, Laureano AU - Cuevas L AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. FAU - Najera, Rafael AU - Najera R AD - Department of Viral Pathogenesis, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain1. LA - eng SI - GENBANK/AF332867 SI - GENBANK/AF408626 SI - GENBANK/AF408627 SI - GENBANK/AF408628 SI - GENBANK/AF408629 SI - GENBANK/AF408630 SI - GENBANK/AF408631 SI - GENBANK/AF408632 PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 RN - 0 (DNA, Viral) RN - 0 (Gene Products, gag) RN - 0 (Gene Products, rev) RN - 0 (HIV Antigens) RN - 0 (HIV Envelope Protein gp41) RN - 0 (Human Immunodeficiency Virus Proteins) RN - 0 (Viral Proteins) RN - 0 (Viral Regulatory and Accessory Proteins) RN - 0 (gag Gene Products, Human Immunodeficiency Virus) RN - 0 (p17 protein, Human Immunodeficiency Virus Type 1) RN - 0 (rev Gene Products, Human Immunodeficiency Virus) RN - 0 (vpu protein, Human immunodeficiency virus 1) RN - EC 2.7.7.49 (HIV Reverse Transcriptase) SB - IM MH - Argentina MH - Base Sequence MH - DNA, Viral MH - Female MH - Gene Products, gag/genetics/physiology MH - Gene Products, rev/genetics/physiology MH - *Genetic Variation MH - *Genome, Viral MH - HIV Antigens/genetics/physiology MH - HIV Envelope Protein gp41/genetics/physiology MH - HIV Infections/*virology MH - HIV Reverse Transcriptase/genetics/physiology MH - HIV-1/classification/*genetics MH - Human Immunodeficiency Virus Proteins MH - Humans MH - Male MH - Molecular Sequence Data MH - *Mosaicism MH - Phylogeny MH - Protein Structure, Tertiary MH - *Recombination, Genetic MH - Sequence Analysis, Protein MH - Sequence Analysis, RNA MH - *Viral Proteins MH - Viral Regulatory and Accessory Proteins/genetics/physiology MH - gag Gene Products, Human Immunodeficiency Virus MH - rev Gene Products, Human Immunodeficiency Virus EDAT- 2001/12/26 10:00 MHDA- 2002/02/20 10:01 CRDT- 2001/12/26 10:00 PHST- 2001/12/26 10:00 [pubmed] PHST- 2002/02/20 10:01 [medline] PHST- 2001/12/26 10:00 [entrez] AID - 10.1099/0022-1317-83-1-107 [doi] PST - ppublish SO - J Gen Virol. 2002 Jan;83(Pt 1):107-119. doi: 10.1099/0022-1317-83-1-107.