PMID- 11753554
OWN - NLM
STAT- MEDLINE
DCOM- 20020927
LR  - 20041117
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 28
IP  - 10
DP  - 2001 Nov
TI  - A rapid monitoring system of human herpesviruses reactivation by LightCycler in 
      stem cell transplantation.
PG  - 975-80
AB  - To establish a practical monitoring system of human herpesviruses reactivation in 
      patients undergoing stem cell transplantation, we developed a new, very rapid, 
      highly sensitive, and quantitative PCR assay for accurate measurement of human 
      cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and Epstein-Barr virus (EBV) 
      DNA using LightCycler. The LightCycler system revealed that there was a linear 
      correlation in the wide range of viral template DNA at the indicated number of 
      PCR cycles. Peripheral blood cells were collected from 16 patients undergoing 
      stem cell transplantation. The cut-off level of CMV and HHV-6 was assessed as 
      10(2) copies/microg and that of EBV as 10(3). High numbers of CMV genomes were 
      detected in 3/13 patients after transplant, and reactivation of HHV-6 was 
      frequently seen, whereas none of the patient showed an elevation of EBV genome 
      copies until the end of the observation period. In the present study, the 
      reactivation of beta herpesviruses is associated with the occurrence of 
      thrombotic microangiopathy (TMA) in two patients undergoing allogeneic BMT. 
      Therefore, it may contribute in clarifying the pathological potential of human 
      herpesviruses using a large number of clinical samples. Our results suggest that 
      this system may be useful for monitoring viral reactivation.
FAU - Aritaki, K
AU  - Aritaki K
AD  - Department of Pediatrics, Tokyo Medical University, Tokyo, Japan.
FAU - Ohyashiki, J H
AU  - Ohyashiki JH
FAU - Suzuki, A
AU  - Suzuki A
FAU - Ojima, T
AU  - Ojima T
FAU - Abe, K
AU  - Abe K
FAU - Shimizu, N
AU  - Shimizu N
FAU - Yamamoto, K
AU  - Yamamoto K
FAU - Ohyashiki, K
AU  - Ohyashiki K
FAU - Hoshika, A
AU  - Hoshika A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cytomegalovirus/genetics/growth & development
MH  - DNA, Viral/blood
MH  - Female
MH  - Gene Dosage
MH  - Hematologic Diseases/therapy/virology
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects
MH  - Herpesviridae/genetics/*growth & development
MH  - Herpesviridae Infections/diagnosis/etiology
MH  - Herpesvirus 4, Human/genetics/growth & development
MH  - Herpesvirus 6, Human/genetics/growth & development
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/instrumentation/*methods/standards
MH  - Time Factors
MH  - Viral Load/methods
MH  - Virus Activation
EDAT- 2001/12/26 10:00
MHDA- 2002/09/28 04:00
CRDT- 2001/12/26 10:00
PHST- 2001/03/27 00:00 [received]
PHST- 2001/08/23 00:00 [accepted]
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/09/28 04:00 [medline]
PHST- 2001/12/26 10:00 [entrez]
AID - 10.1038/sj.bmt.1703266 [doi]
PST - ppublish
SO  - Bone Marrow Transplant. 2001 Nov;28(10):975-80. doi: 10.1038/sj.bmt.1703266.