PMID- 11754092 OWN - NLM STAT- MEDLINE DCOM- 20020312 LR - 20061115 IS - 0739-4462 (Print) IS - 0739-4462 (Linking) VI - 49 IP - 1 DP - 2002 Jan TI - Detection of a single copy gene on a mitotic metaphase chromosome by fluorescence in situ hybridization (FISH) in the sawfly, Athalia rosae (Hymenoptera). PG - 34-40 AB - Mitotic metaphase chromosomes of Athalia rosae (Hymenoptera) haploid males were subjected to fluorescence in situ hybridization (FISH) analysis using an rDNA probe and two vitellogenin (Vg) cDNA probes (one representing the 5' half and the other the 3' half of the gene, each about 3 kb long, and together covering the entire coding region). The rDNA probe produced signals in four chromosomes, all in pericentromeric regions (haploid chromosome number = 8), and the Vg probes, either the combined probes or the 3' region alone, produced a twin signal in the middle of a chromosome arm of a single chromosome. Arch. CI - Copyright 2002 Wiley-Liss, Inc. FAU - Matsumoto, Kouhei AU - Matsumoto K AD - Division of Biology, Graduate School of Science and Technology, Kobe University, Nada, Kobe, Japan. FAU - Yamamoto, Daisuke S AU - Yamamoto DS FAU - Sumitani, Megumi AU - Sumitani M FAU - Lee, Jae Min AU - Lee JM FAU - Hatakeyama, Masatsugu AU - Hatakeyama M FAU - Oishi, Kugao AU - Oishi K LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Arch Insect Biochem Physiol JT - Archives of insect biochemistry and physiology JID - 8501752 RN - 0 (DNA Probes) RN - 0 (DNA, Complementary) RN - 0 (DNA, Ribosomal) RN - 0 (Vitellogenins) SB - IM MH - Animals MH - Blotting, Southern MH - Chromosome Mapping/methods MH - Chromosomes/*genetics MH - DNA Probes MH - DNA, Complementary/genetics MH - DNA, Ribosomal/chemistry/genetics MH - *Gene Dosage MH - Hymenoptera/*genetics MH - In Situ Hybridization, Fluorescence MH - Male MH - Metaphase/genetics MH - Vitellogenins/chemistry/genetics EDAT- 2001/12/26 10:00 MHDA- 2002/03/13 10:01 CRDT- 2001/12/26 10:00 PHST- 2001/12/26 10:00 [pubmed] PHST- 2002/03/13 10:01 [medline] PHST- 2001/12/26 10:00 [entrez] AID - 10.1002/arch.10005 [pii] AID - 10.1002/arch.10005 [doi] PST - ppublish SO - Arch Insect Biochem Physiol. 2002 Jan;49(1):34-40. doi: 10.1002/arch.10005.