PMID- 11754530
OWN - NLM
STAT- MEDLINE
DCOM- 20020426
LR  - 20220311
IS  - 0273-2300 (Print)
IS  - 0273-2300 (Linking)
VI  - 34
IP  - 3
DP  - 2001 Dec
TI  - ICCVAM evaluation of the murine local lymph node assay. Conclusions and 
      recommendations of an independent scientific peer review panel.
PG  - 258-73
AB  - The validation status of the murine local lymph node assay (LLNA), a method for 
      assessing the allergic contact dermatitis potential of chemicals, was evaluated 
      by an independent peer review panel (Panel) convened by the Interagency 
      Coordinating Committee on the Validation of Alternative Methods (ICCVAM). The 
      LLNA measures lymphocyte proliferation using incorporation of radioactive 
      thymidine or iododeoxyuridine into cells of the draining lymph nodes of mice 
      topically exposed to a test article. The Panel concluded that the assay performed 
      as well as currently accepted guinea pig methods [guinea pig maximization test 
      (GPMT)/Buehler assay (BA)] for the hazard identification of strong to moderate 
      chemical sensitizing agents, but that it might not correctly identify all weak 
      sensitizers or metals (potential false negative response) or all strong irritants 
      (potential false positive response). The Panel concluded also that the LLNA 
      involves less pain and distress than conventional guinea pig methods. The Panel 
      unanimously recommended the LLNA as a stand-alone alternative for contact 
      sensitization hazard assessment, provided that certain protocol modifications 
      were made. These included collection of individual, rather than pooled, animal 
      response data; the inclusion of a concurrent positive control; and consideration 
      of dose-response information and statistical analyses. A standardized LLNA 
      protocol is provided.
FAU - Dean, J H
AU  - Dean JH
AD  - Sanofi-Synthelabo Research, Sanofi-Synthelabo Inc., Malvern, PA 19355, USA.
FAU - Twerdok, L E
AU  - Twerdok LE
FAU - Tice, R R
AU  - Tice RR
FAU - Sailstad, D M
AU  - Sailstad DM
FAU - Hattan, D G
AU  - Hattan DG
FAU - Stokes, W S
AU  - Stokes WS
LA  - eng
GR  - N01-ES-85424/ES/NIEHS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Regul Toxicol Pharmacol
JT  - Regulatory toxicology and pharmacology : RTP
JID - 8214983
RN  - LGP81V5245 (Idoxuridine)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Animals
MH  - Dermatitis, Allergic Contact/diagnosis/*etiology
MH  - Environmental Exposure/*standards
MH  - Female
MH  - Government Agencies/standards
MH  - Guideline Adherence
MH  - Guidelines as Topic/standards
MH  - Guinea Pigs
MH  - Humans
MH  - Idoxuridine/administration & dosage
MH  - *Interinstitutional Relations
MH  - Isotope Labeling
MH  - *Local Lymph Node Assay
MH  - Lymphocytes/physiology
MH  - Mice
MH  - Peer Review/methods/standards
MH  - Reproducibility of Results
MH  - Risk Assessment
MH  - Thymidine/administration & dosage
MH  - Toxicity Tests/methods/*standards
MH  - United States
EDAT- 2002/01/05 10:00
MHDA- 2002/04/27 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/04/27 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - S0273230001914977 [pii]
AID - 10.1006/rtph.2001.1497 [doi]
PST - ppublish
SO  - Regul Toxicol Pharmacol. 2001 Dec;34(3):258-73. doi: 10.1006/rtph.2001.1497.