PMID- 11754531
OWN - NLM
STAT- MEDLINE
DCOM- 20020426
LR  - 20071115
IS  - 0273-2300 (Print)
IS  - 0273-2300 (Linking)
VI  - 34
IP  - 3
DP  - 2001 Dec
TI  - ICCVAM evaluation of the murine local lymph node assay. Data analyses completed 
      by the National Toxicology Program Interagency Center for the Evaluation of 
      Alternative Toxicological Methods.
PG  - 274-86
AB  - To evaluate the reliability of the murine local lymph node assay (LLNA), a test 
      for allergic contact dermatitis activity, the inter- and intralaboratory 
      consistency statistics (h and k, respectively) were calculated for validation 
      studies testing multiple chemicals. The analysis indicated the absence of 
      excessive variability in the dose calculated to induce a threefold or greater 
      increase in the stimulation index (SI). To assess the appropriateness of using an 
      SI of 3 as the decision criteria for identifying a sensitizing compound, LLNA 
      results based on SI values of 2.0, 2.5, 3.0, 3.5, and 4.0 were compared with 
      guinea pig or human results. The results supported the use of an SI of 3 as the 
      decision criteria. Assay performance was determined by comparing LLNA results to 
      results obtained for guinea pigs or humans. The accuracy of the LLNA was 89% when 
      compared with results from the guinea pig maximization test (GPMT)/Buehler assay 
      (BA). The performance of the LLNA and the GPMT/BA was similar when each was 
      compared to human maximization test results plus substances included as human 
      patch test allergens. The LLNA offered advantages over the GPMT in respect to 
      both the time required to conduct the test and the assay cost.
FAU - Haneke, K E
AU  - Haneke KE
AD  - National Toxicology Program Interagency Center for the Evaluation of Alternative 
      Toxicological Methods, National Institute of Environmental Health Sciences, MD 
      EC-17, Research Triangle Park, NC 27709, USA.
FAU - Tice, R R
AU  - Tice RR
FAU - Carson, B L
AU  - Carson BL
FAU - Margolin, B H
AU  - Margolin BH
FAU - Stokes, W S
AU  - Stokes WS
LA  - eng
GR  - N01-ES-85424/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Regul Toxicol Pharmacol
JT  - Regulatory toxicology and pharmacology : RTP
JID - 8214983
RN  - 0 (Organic Chemicals)
SB  - IM
MH  - Animal Welfare
MH  - Animals
MH  - Dermatitis, Allergic Contact/diagnosis/economics/*etiology
MH  - Environmental Exposure/economics/statistics & numerical data
MH  - Government Agencies/economics/statistics & numerical data
MH  - Guideline Adherence
MH  - Guidelines as Topic/standards
MH  - Guinea Pigs
MH  - Humans
MH  - *Interinstitutional Relations
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/drug effects
MH  - Mice
MH  - Organic Chemicals/analysis/*toxicity
MH  - Peer Review/methods/standards
MH  - Reproducibility of Results
MH  - Risk Assessment/economics/statistics & numerical data
MH  - Sensitivity and Specificity
MH  - Toxicity Tests/economics/methods/*statistics & numerical data
MH  - United States
EDAT- 2002/01/05 10:00
MHDA- 2002/04/27 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/04/27 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - S0273230001914989 [pii]
AID - 10.1006/rtph.2001.1498 [doi]
PST - ppublish
SO  - Regul Toxicol Pharmacol. 2001 Dec;34(3):274-86. doi: 10.1006/rtph.2001.1498.