PMID- 11755384
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20191105
IS  - 1056-8719 (Print)
IS  - 1056-8719 (Linking)
VI  - 45
IP  - 3
DP  - 2001 May-Jun
TI  - Validation of the use of field effect transistors for extracellular signal 
      recording in pharmacological bioassays.
PG  - 207-14
AB  - The use of field effect transistors (FETs) in biomedical research has been in 
      rapid progression in recent years. The present study aims to demonstrate a 
      quantitative use of these devices in pharmacological bioassays. FETs were made as 
      a 4 x 4 matrix of gates with a width of 200 microm separating each gate. The 
      surface of the FETs (silicon oxide), covered with a layer of laminin, 
      fibronectin, or nitro cellulose was suitable for cell adhesion. The cultured 
      dissociated cardiac myocytes were spontaneously active within 24 to 48 h after 
      initial plating. Simultaneous intracellular patch clamp recordings were used to 
      verify the electrophysiological signals of cells that were coupled to the gates. 
      All positive chronotropes (isoproterenol, norepinephrine) and negative 
      chronotropes (verapamil, carbamylcholine, SDZ PCO400) showed their characteristic 
      effects on heart cells in terms of changes of beat frequency. As the myocytes 
      were in a complete syncitium on each FET, the cells need not be directly coupled 
      to the gate in order to detect any ionic changes. This enables global cellular 
      responses to be analyzed. The system also offers an opportunity to study the 
      interconnections and communications between different cells. Furthermore, the 
      changes of signal shapes in the presence of different agents could also be 
      detected. The present study demonstrates how versatile and sensitive this 
      recording system is in distinguishing different ionic signal shapes. The authors 
      believe that this system has the potential to replace some currently employed in 
      vitro methods, offering an alternative, which can substantially reduce animal use 
      in pharmacological experiments.
FAU - Yeung, C K
AU  - Yeung CK
AD  - Max-Planck-Institute for Polymer Research, Ackermannweg 10, Mainz, D-55128, 
      Germany. ckyeung@dera.gov.uk
FAU - Ingebrandt, S
AU  - Ingebrandt S
FAU - Krause, M
AU  - Krause M
FAU - Offenhausser, A
AU  - Offenhausser A
FAU - Knoll, W
AU  - Knoll W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pharmacol Toxicol Methods
JT  - Journal of pharmacological and toxicological methods
JID - 9206091
RN  - 0 (Benzopyrans)
RN  - 0 (Cardiovascular Agents)
RN  - 0 (Cyclopentanes)
RN  - 121055-10-5 (SDZ PCO 400)
RN  - 8Y164V895Y (Carbachol)
RN  - CJ0O37KU29 (Verapamil)
RN  - L628TT009W (Isoproterenol)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Action Potentials
MH  - Animals
MH  - Benzopyrans/pharmacology
MH  - Biological Assay/*instrumentation/methods
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Carbachol/pharmacology
MH  - Cardiovascular Agents/pharmacology
MH  - Cells, Cultured
MH  - Cyclopentanes/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Electrochemistry/instrumentation
MH  - Electrodes
MH  - Heart/drug effects/embryology/physiology
MH  - Isoproterenol/pharmacology
MH  - Myocardium/cytology
MH  - Norepinephrine/pharmacology
MH  - Patch-Clamp Techniques
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Semiconductors
MH  - Transistors, Electronic
MH  - Verapamil/pharmacology
EDAT- 2002/01/05 10:00
MHDA- 2002/03/01 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - S1056-8719(01)00150-2 [pii]
AID - 10.1016/s1056-8719(01)00150-2 [doi]
PST - ppublish
SO  - J Pharmacol Toxicol Methods. 2001 May-Jun;45(3):207-14. doi: 
      10.1016/s1056-8719(01)00150-2.