PMID- 11755467
OWN - NLM
STAT- MEDLINE
DCOM- 20020327
LR  - 20201208
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 26
IP  - 2
DP  - 2002 Feb
TI  - Interferon-alpha 2b and vesnarinone influence levels of tumor necrosis 
      factor-alpha, apoptosis, or interleukin 6 in ESKOL, a hairy cell leukemic cell 
      line. A potential cytokine and oncogene relationship regulating apoptosis is 
      suggested.
PG  - 169-77
AB  - The in vitro effects of interferon-alpha (IFN) on levels of secreted interleukin 
      6 (IL-6), tumor necrosis factor-alpha (TNF alpha), and nuclear matrix proteins 
      (NMP) were examined in ESKOL, a B-lymphoblastoid cell line resembling hairy cell 
      leukemia (HCL). IFN enhances differentiation in ESKOL, decreases TNF alpha 
      levels, decreases apoptosis, increases IL-6 levels, and down regulates the 
      expression of several oncogenes. Vesnarinone (Ves), a TNF alpha repressor, lowers 
      TNF-alpha and decreases apoptosis in the same cell line. ESKOL exhibits enhanced 
      apoptosis and reduced B-cell lymphomas (Bcl-2) levels over WIL-2. IL-6 and 
      TNFalpha have been shown to decrease and increase apoptosis in B-cells 
      respectively; however, treatment of ESKOL with these cytokines had no significant 
      effect on apoptosis. We suggest that IFN decreases apoptosis by mechanisms 
      involving enhanced IL-6 and Bcl-2 levels, decreased TNF alpha and the down 
      regulation of apoptotic oncogenes, including c-myc.
FAU - Khalaf, Waleed
AU  - Khalaf W
AD  - Department of Microbiology and Immunology, Indiana University School of Medicine, 
      Indianapolis, IN 46222, USA.
FAU - Maina, Caroline
AU  - Maina C
FAU - Byers, Joseph
AU  - Byers J
FAU - Harvey, William
AU  - Harvey W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Interferon alpha-2)
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-6)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Pyrazines)
RN  - 0 (Quinolines)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 5COW40EV8M (vesnarinone)
SB  - IM
CIN - Leuk Res. 2002 Apr;26(4):407-8. PMID: 11839386
MH  - Antigens, Nuclear
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects/genetics
MH  - Gene Expression Regulation, Leukemic/*drug effects
MH  - Genes, bcl-2
MH  - Humans
MH  - Interferon alpha-2
MH  - Interferon-alpha/*pharmacology
MH  - Interleukin-6/*biosynthesis/metabolism
MH  - Leukemia, Hairy Cell/genetics/metabolism/*pathology
MH  - Nuclear Proteins/biosynthesis/genetics
MH  - Oncogenes
MH  - Proto-Oncogene Proteins c-bcl-2/biosynthesis
MH  - Pyrazines
MH  - Quinolines/*pharmacology
MH  - Recombinant Proteins
MH  - Tumor Cells, Cultured/drug effects/metabolism
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/metabolism
EDAT- 2002/01/05 10:00
MHDA- 2002/03/28 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/03/28 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - S014521260100100X [pii]
AID - 10.1016/s0145-2126(01)00100-x [doi]
PST - ppublish
SO  - Leuk Res. 2002 Feb;26(2):169-77. doi: 10.1016/s0145-2126(01)00100-x.