PMID- 11755469
OWN - NLM
STAT- MEDLINE
DCOM- 20020327
LR  - 20190826
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 26
IP  - 2
DP  - 2002 Feb
TI  - Optimal cytokine stimulation for the enhanced generation of leukemic dendritic 
      cells in short-term culture.
PG  - 191-201
AB  - Dendritic cells (DCs) are professional antigen presenting cells derived from 
      myeloid or lymphoid precursors. Functional DCs have been generated from the 
      malignant counterpart of these precursor cells. Herein, we describe the 
      generation of DCs from different leukemias and determine the optimal culture 
      conditions with minimal manipulation. Primary leukemic cells were cultured for 1, 
      3, and 5 days in 11 different cytokine combinations and analyzed for the 
      expression of a mature DC phenotype. Optimal growth and DC characteristics were 
      obtained with GM-CSF, FL, and SCF in 3-5 day cultures, suggesting a practical 
      strategy for the immunotherapy of leukemia.
FAU - Panoskaltsis, Nicki
AU  - Panoskaltsis N
AD  - The University of Rochester Medical Center, Department of Internal Medicine, 
      Hematology/Oncology Unit, Box 610, Rochester, NY 14642, USA. 
      n.panoskaltsis@ic.ac.uk
FAU - Belanger, Todd J
AU  - Belanger TJ
FAU - Liesveld, Jane L
AU  - Liesveld JL
FAU - Abboud, Camille N
AU  - Abboud CN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Cytokines)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Stem Cell Factor)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1)
SB  - IM
CIN - Leuk Res. 2002 Apr;26(4):409-10. PMID: 11839387
MH  - Acute Disease
MH  - Aged
MH  - Cell Count
MH  - Cell Differentiation/drug effects
MH  - Child, Preschool
MH  - Cytokines/*pharmacology
MH  - Dendritic Cells/*cytology
MH  - Female
MH  - Flow Cytometry
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia/*pathology
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/pathology
MH  - Leukemia, Myeloid/pathology
MH  - Leukemia, Myelomonocytic, Chronic/pathology
MH  - Male
MH  - Microscopy, Phase-Contrast
MH  - Middle Aged
MH  - Neoplastic Stem Cells/cytology/*drug effects
MH  - Phenotype
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/pathology
MH  - Proto-Oncogene Proteins/pharmacology
MH  - Receptor Protein-Tyrosine Kinases/pharmacology
MH  - Recombinant Fusion Proteins/pharmacology
MH  - Stem Cell Factor/pharmacology
MH  - T-Lymphocytes/immunology
MH  - Tumor Cells, Cultured/drug effects
MH  - Vascular Endothelial Growth Factor Receptor-1
EDAT- 2002/01/05 10:00
MHDA- 2002/03/28 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/03/28 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - S0145212601001047 [pii]
AID - 10.1016/s0145-2126(01)00104-7 [doi]
PST - ppublish
SO  - Leuk Res. 2002 Feb;26(2):191-201. doi: 10.1016/s0145-2126(01)00104-7.