PMID- 11756323
OWN - NLM
STAT- MEDLINE
DCOM- 20020213
LR  - 20211203
IS  - 0012-1797 (Print)
IS  - 0012-1797 (Linking)
VI  - 51
IP  - 1
DP  - 2002 Jan
TI  - Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet 
      transplantation.
PG  - 55-65
AB  - We investigated the capacity of human islets to produce monocyte chemoattractant 
      protein-1 (MCP-1). Primary cultures of pancreatic islets expressed and secreted 
      MCP-1, as determined by Northern blot, immunohistochemistry, in situ 
      hybridization, and enzyme-linked immunosorbent assay. The produced MCP-1 was 
      biologically active as it attracted monocytes in chemotaxis assay, and 
      chemotactic activity was almost abrogated by a neutralizing anti-MCP-1 monoclonal 
      antibody. Expression of MCP-1 was increased by primary inflammatory cytokines 
      (interleukin-1 beta, tumor necrosis factor-alpha) and lipopolysaccharide at both 
      the mRNA and protein levels but not by glucose. However, MCP-1 did not modulate 
      insulin secretion. MCP-1 secreted by pancreatic islets plays a relevant role in 
      the clinical outcome of islet transplant in patients with type 1 diabetes. In 
      fact, low MCP-1 secretion resulted as the most relevant factor for long-lasting 
      insulin independence. This finding opens new approaches in the management of 
      human islet transplantation. Finally, the finding that MCP-1 appears 
      constitutively present in normal human islet beta-cells (immunohistochemistry and 
      in situ hybridization), in the absence of an inflammatory infiltrate, suggests 
      that this chemokine could have functions other than monocyte recruitment and 
      opens a new link between the endocrine and immune systems.
FAU - Piemonti, Lorenzo
AU  - Piemonti L
AD  - Laboratory of Experimental Surgery, Surgical Department, S. Raffaele Scientific 
      Institute, Via Olgettina, Milan, Italy. University of Milano Bicocca, Milan, 
      Italy. piemonti.lorenzo@hsr.it
FAU - Leone, Biagio Eugenio
AU  - Leone BE
FAU - Nano, Rita
AU  - Nano R
FAU - Saccani, Alessandra
AU  - Saccani A
FAU - Monti, Paolo
AU  - Monti P
FAU - Maffi, Paola
AU  - Maffi P
FAU - Bianchi, Giancarlo
AU  - Bianchi G
FAU - Sica, Antonio
AU  - Sica A
FAU - Peri, Giuseppe
AU  - Peri G
FAU - Melzi, Raffaella
AU  - Melzi R
FAU - Aldrighetti, Luca
AU  - Aldrighetti L
FAU - Secchi, Antonio
AU  - Secchi A
FAU - Di Carlo, Valerio
AU  - Di Carlo V
FAU - Allavena, Paola
AU  - Allavena P
FAU - Bertuzzi, Federico
AU  - Bertuzzi F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diabetes
JT  - Diabetes
JID - 0372763
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Insulin)
SB  - IM
MH  - Adult
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics/metabolism/pharmacology
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Cytokines/pharmacology
MH  - Diabetes Mellitus, Type 1/*surgery
MH  - Diabetic Nephropathies/surgery
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Immunosuppression Therapy
MH  - Insulin/*metabolism
MH  - Insulin Secretion
MH  - Islets of Langerhans/*metabolism
MH  - *Islets of Langerhans Transplantation/*physiology
MH  - Kidney Transplantation
MH  - Male
MH  - Middle Aged
MH  - Monocytes/physiology
EDAT- 2002/01/05 10:00
MHDA- 2002/02/14 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/02/14 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.2337/diabetes.51.1.55 [doi]
PST - ppublish
SO  - Diabetes. 2002 Jan;51(1):55-65. doi: 10.2337/diabetes.51.1.55.