PMID- 11756473
OWN - NLM
STAT- MEDLINE
DCOM- 20020130
LR  - 20220129
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 155
IP  - 7
DP  - 2001 Dec 24
TI  - Small cargo proteins and large aggregates can traverse the Golgi by a common 
      mechanism without leaving the lumen of cisternae.
PG  - 1225-38
AB  - Procollagen (PC)-I aggregates transit through the Golgi complex without leaving 
      the lumen of Golgi cisternae. Based on this evidence, we have proposed that PC-I 
      is transported across the Golgi stacks by the cisternal maturation process. 
      However, most secretory cargoes are small, freely diffusing proteins, thus 
      raising the issue whether they move by a transport mechanism different than that 
      used by PC-I. To address this question we have developed procedures to compare 
      the transport of a small protein, the G protein of the vesicular stomatitis virus 
      (VSVG), with that of the much larger PC-I aggregates in the same cell. Transport 
      was followed using a combination of video and EM, providing high resolution in 
      time and space. Our results reveal that PC-I aggregates and VSVG move 
      synchronously through the Golgi at indistinguishable rapid rates. Additionally, 
      not only PC-I aggregates (as confirmed by ultrarapid cryofixation), but also 
      VSVG, can traverse the stack without leaving the cisternal lumen and without 
      entering Golgi vesicles in functionally relevant amounts. Our findings indicate 
      that a common mechanism independent of anterograde dissociative carriers is 
      responsible for the traffic of small and large secretory cargo across the Golgi 
      stack.
FAU - Mironov, A A
AU  - Mironov AA
AD  - Department of Cell Biology and Oncology, Istituto di Ricerche Farmacologiche 
      "Mario Negri," 66030 Santa Maria Imbaro, Chieti, Italy.
FAU - Beznoussenko, G V
AU  - Beznoussenko GV
FAU - Nicoziani, P
AU  - Nicoziani P
FAU - Martella, O
AU  - Martella O
FAU - Trucco, A
AU  - Trucco A
FAU - Kweon, H S
AU  - Kweon HS
FAU - Di Giandomenico, D
AU  - Di Giandomenico D
FAU - Polishchuk, R S
AU  - Polishchuk RS
FAU - Fusella, A
AU  - Fusella A
FAU - Lupetti, P
AU  - Lupetti P
FAU - Berger, E G
AU  - Berger EG
FAU - Geerts, W J
AU  - Geerts WJ
FAU - Koster, A J
AU  - Koster AJ
FAU - Burger, K N
AU  - Burger KN
FAU - Luini, A
AU  - Luini A
LA  - eng
GR  - E.0982/TI_/Telethon/Italy
GR  - E.1105/TI_/Telethon/Italy
GR  - E.1249/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20011224
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Antibodies)
RN  - 0 (G protein, vesicular stomatitis virus)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Cell Line
MH  - Fibroblasts/*metabolism/ultrastructure
MH  - Freezing
MH  - Golgi Apparatus/*metabolism/ultrastructure
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Luminescent Proteins/genetics/metabolism
MH  - *Membrane Glycoproteins
MH  - Microscopy, Electron
MH  - Microscopy, Immunoelectron
MH  - *Protein Transport
MH  - Rabbits
MH  - Recombinant Proteins/metabolism
MH  - Skin/metabolism/ultrastructure
MH  - *Skin Physiological Phenomena
MH  - Viral Envelope Proteins/metabolism
PMC - PMC2199327
EDAT- 2002/01/05 10:00
MHDA- 2002/01/31 10:01
PMCR- 2002/06/24
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/01/31 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PHST- 2002/06/24 00:00 [pmc-release]
AID - jcb.200108073 [pii]
AID - 0108073 [pii]
AID - 10.1083/jcb.200108073 [doi]
PST - ppublish
SO  - J Cell Biol. 2001 Dec 24;155(7):1225-38. doi: 10.1083/jcb.200108073. Epub 2001 
      Dec 24.