PMID- 11756573
OWN - NLM
STAT- MEDLINE
DCOM- 20020709
LR  - 20190513
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 8
IP  - 1
DP  - 2002 Jan
TI  - Novel hepatocyte growth factor/scatter factor isoform transcripts in the macaque 
      endometrium and placenta.
PG  - 81-7
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) induces proliferation, motility 
      and morphogenesis of cells that express the proto-oncogene for the tyrosine 
      kinase receptor, c-Met. Because these cellular events occur in the endometrium 
      during the menstrual cycle and in placenta during development, we have initiated 
      studies of this growth factor in these tissues from macaques. Several HGF/SF 
      alternatively spliced transcripts have been previously reported in other tissues. 
      However, expression of HGF/SF isoforms in the endometrium has not been studied. 
      Here we describe the relative transcript amounts of HGF/SF isoforms in the 
      endometrium and placenta using RNase protection analyses. During these analyses, 
      we discovered two unexpected protected bands that were found through sequence 
      analyses to represent isoforms similar to the previously reported NK1 and NK2 
      except that they encode a five amino acid deletion in the first kringle domain. 
      We designated these two isoforms as dNK1 and dNK2. Endometrium expressed all of 
      the isoforms; however, dNK2 was consistently expressed at higher levels than NK2 
      transcripts. In contrast, placenta expressed NK2 and dNK2 mRNA at equal levels, 
      and both NK1 and dNK1 were undetectable in placenta. HGF/SF function in 
      endometrium and placenta may involve complex interactions between the isoforms of 
      HGF/SF and those of c-Met.
FAU - Lindsey, J Suzanne
AU  - Lindsey JS
AD  - Pharmaceutical Sciences Department, Texas Tech University Health Science Center 
      School of Pharmacy, 1300 Coulter Drive, Amarillo, TX 79106, USA. 
      suzanne@cortex.ama.ttuhsc.edu
FAU - Brenner, Robert M
AU  - Brenner RM
LA  - eng
GR  - HD 19182/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Protein Isoforms)
RN  - 63231-63-0 (RNA)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Alternative Splicing
MH  - Animals
MH  - Base Sequence
MH  - Endometrium/*physiology
MH  - Female
MH  - Hepatocyte Growth Factor/genetics/*metabolism
MH  - Macaca/*physiology
MH  - Macaca mulatta/physiology
MH  - Macaca nemestrina/physiology
MH  - Menstrual Cycle/physiology
MH  - Molecular Sequence Data
MH  - Placenta/*physiology
MH  - Protein Isoforms
MH  - Proto-Oncogene Proteins c-met/metabolism
MH  - RNA/metabolism
EDAT- 2002/01/05 10:00
MHDA- 2002/07/10 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/07/10 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1093/molehr/8.1.81 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2002 Jan;8(1):81-7. doi: 10.1093/molehr/8.1.81.