PMID- 11757427
OWN - NLM
STAT- MEDLINE
DCOM- 20020326
LR  - 20061115
IS  - 0025-8601 (Print)
IS  - 0025-8601 (Linking)
VI  - 53
IP  - 2
DP  - 2001
TI  - [Analysis of fatty acids from lipopolysaccharides of Bacteroides thetaiotaomicron 
      and Bacteroides fragilis].
PG  - 177-83
AB  - The aim of this study was to determine and to compare fatty acids occurring in 
      lipopolysaccharides (LPS) isolated from B. thetaiotaomicron and B. fragilis 
      strains of different origin. Lipopolysaccharides of three B. thetaiotaomicron 
      strains and four B. fragilis strains were isolated by phenol-water extraction 
      according to the procedure of Westphal and Jann (1965). Water-phase LPS fractions 
      were then treated with nucleases and purified by ultracentrifugation as described 
      by Gmeiner (1975). Fatty acid methyl esters, obtained by methanolysis of LPS, 
      were analysed in gas-liquid chromatography combined with mass spectrometry 
      (GLC-MS). Trimethylsilylated hydroxyl groups of fatty acid methyl esters were 
      identified with GLC-MS using a method of selective ion monitoring (SIM). 
      Lipopolysaccharides of B. thetaiotaomicron and B. fragilis strains contained 
      long-chain (15-18 carbon atoms) fatty acids. The broad spectrum of simple 
      long-chain and branched-chain fatty acids as well as 3-hydroxy fatty acids were 
      detected. The main fatty acid of analyzed bacterial species was 
      3-hydroxy-hexadecanoic acid (3OH C16:0). Several 3-hydroxy fatty acids were 
      detected in LPS of examined strains. Fatty acids occurring in LPS of B. 
      thetaiotaomicron and B. fragilis strains appeared to be qualitatively similar. 
      Quantitative differences in fatty acids composition of lipopolysaccharides 
      isolated from strains of different origin were observed.
FAU - Rokosz, A
AU  - Rokosz A
AD  - Katedra i Zaklad Mikrobiologii Lekarskiej AM w Warszawie.
FAU - Meisel-Mikolajczyk, F
AU  - Meisel-Mikolajczyk F
FAU - Kot, K
AU  - Kot K
FAU - Mieszala, M
AU  - Mieszala M
FAU - Szponar, B
AU  - Szponar B
FAU - Gamian, A
AU  - Gamian A
LA  - pol
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
TT  - Analiza kwasow tluszczowych wystepujacych w lipopolisacharydach Bacteroides 
      thetaiotaomicron i Bacteroides fragilis.
PL  - Poland
TA  - Med Dosw Mikrobiol
JT  - Medycyna doswiadczalna i mikrobiologia
JID - 0210575
RN  - 0 (Fatty Acids)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Bacteroides/*chemistry/*classification
MH  - Bacteroides fragilis/chemistry
MH  - Fatty Acids/*analysis
MH  - Humans
MH  - Lipopolysaccharides/*analysis
MH  - Species Specificity
EDAT- 2002/01/05 10:00
MHDA- 2002/03/27 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/03/27 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Med Dosw Mikrobiol. 2001;53(2):177-83.