PMID- 11759063
OWN - NLM
STAT- MEDLINE
DCOM- 20030512
LR  - 20191105
IS  - 1541-2016 (Print)
IS  - 1533-4058 (Linking)
VI  - 9
IP  - 4
DP  - 2001 Dec
TI  - A reliable method of demonstrating HER-2/neu, estrogen receptors, and 
      progesterone receptors on routinely processed cytologic material.
PG  - 352-7
AB  - Estrogen (ER) and progesterone receptors (PR) and HER-2/neu (c-erbB-2) protein 
      overexpression are important prognostic factors for breast carcinoma. This study 
      describes a simple, rapid method that demonstrates the applicability of these 
      tests to cytology smears. Smears were made from scrape samples of fresh breast 
      tumors and were fixed in 95% alcohol for immunohistochemical staining for ER and 
      PR and HER-2/neu protein overexpression. Scraped material was suspended in 
      phosphate-buffered saline, and cytospin slides were prepared from the suspension 
      and air dried for fluorescence in situ hybridization (FISH). The results for ER 
      performed on cytology compared with the gold standard (immunohistochemistry on 
      histology) showed 94% accuracy (95% confidence bounds (0.8432 0.9823)); PR 
      results showed 71% accuracy (95% confidence bounds (0.5805 0.8180)) and HER-2/neu 
      results showed 77% accuracy (95% confidence bounds (0.6500 0.8709)). Using 
      HER-2/neu gene overamplification detected by FISH as the gold standard, 
      immunohistochemistry for HER-2/neu protein overexpression showed sensitivity, 
      specificity, and a positive predictive value of 88.8%, 77.7%, and 80% for 
      histology, respectively, and 69%, 87%, and 90% for cytology, respectively. This 
      study showed that fixation and storage of smears in 95% alcohol were effective 
      for receptor studies, whereas air-dried cytospin smears were well suited to FISH.
FAU - McKee, G T
AU  - McKee GT
AD  - Department of Cytopathology, Massachusetts General Hospital, Boston 02493, USA. 
      gmckee@partners.org
FAU - Tambouret, R H
AU  - Tambouret RH
FAU - Finkelstein, D
AU  - Finkelstein D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/chemistry/diagnosis/*pathology
MH  - Cytological Techniques/methods
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/standards
MH  - In Situ Hybridization, Fluorescence/standards
MH  - Predictive Value of Tests
MH  - Receptor, ErbB-2/*analysis
MH  - Receptors, Estrogen/*analysis
MH  - Receptors, Progesterone/*analysis
MH  - Sensitivity and Specificity
EDAT- 2002/01/05 10:00
MHDA- 2003/05/13 05:00
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2003/05/13 05:00 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1097/00129039-200112000-00011 [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2001 Dec;9(4):352-7. doi: 
      10.1097/00129039-200112000-00011.