PMID- 11759073
OWN - NLM
STAT- MEDLINE
DCOM- 20020514
LR  - 20201219
IS  - 1524-9557 (Print)
IS  - 1524-9557 (Linking)
VI  - 24
IP  - 6
DP  - 2001 Nov-Dec
TI  - Interactions between dendritic cells and cytokine-induced killer cells lead to an 
      activation of both populations.
PG  - 502-10
AB  - Dendritic cells (DCs) are major antigen-presenting cells. They are capable of 
      capturing and processing tumor antigens, expressing lymphocyte costimulatory 
      molecules, and secreting cytokines to initiate immune responses. Here, the 
      authors tested the effect of cytokine-induced killer (CIK) cells, a population 
      that includes CD3+CD56+ cells (natural killer T cells), with regard to their 
      capacity to immunomodulate DCs. Cytokine-induced killer cells were cocultured 
      with autologous DCs generated from peripheral blood mononuclear cells. Expression 
      of markers typical for both populations was measured using flow cytometry, and 
      secretion of interleukin (IL)-12 was determined using enzyme-linked immunosorbent 
      assays. Cytotoxicity assays were performed to investigate the role of IL-12 and 
      the importance of cell-cell interactions. Considering this, receptors for IL-12 
      and CD40 were blocked and cocultures were performed with cell culture inserts. 
      Coculture of CIK cells led to a significant increase of DC-specific, 
      costimulatory, and antigen-presenting molecules in DC cultures. In addition, 
      coculture resulted in a dramatically increase of IL-12 secretion by DCs and to a 
      significant increase in cytotoxic activity of CIK cells toward carcinoma cells. 
      Blockage of IL-12 uptake decreased the cytolytic activity of CIK cells. Cytokine 
      secretion was shown to be important for activation of CIK cells, and also 
      cellular interactions between DCs and effector cells caused a higher cytolytic 
      capacity. Interactions between DCs and CIK cells caused changes in the surface 
      molecule expression of both populations, led to an increase of IL-12 secretion, 
      and rendered an improved cytotoxic activity. The natural killer T cell 
      subpopulation seems to be responsible for this effect. Therefore, coculture of 
      DCs with CIK cells may have a major impact on immunotherapeutic protocols for 
      patients with cancer.
FAU - Marten, A
AU  - Marten A
AD  - Medizinische Klinik und Poliklinik I, Allgemeine Innere Medizin, Rheinische 
      Friedrich-Wilhelms-Universitat, Bonn, Germany.
FAU - Ziske, C
AU  - Ziske C
FAU - Schottker, B
AU  - Schottker B
FAU - Renoth, S
AU  - Renoth S
FAU - Weineck, S
AU  - Weineck S
FAU - Buttgereit, P
AU  - Buttgereit P
FAU - Schakowski, F
AU  - Schakowski F
FAU - von Rucker, A
AU  - von Rucker A
FAU - Sauerbruch, T
AU  - Sauerbruch T
FAU - Schmidt-Wolf, I G
AU  - Schmidt-Wolf IG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunother
JT  - Journal of immunotherapy (Hagerstown, Md. : 1997)
JID - 9706083
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-12)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Biomarkers
MH  - Cell Communication/immunology
MH  - Cell Membrane/immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytokines/*immunology
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Interleukin-12/metabolism
MH  - Killer Cells, Natural/*immunology
MH  - Lymphocyte Activation/*immunology
MH  - Receptors, Interleukin/immunology
MH  - Receptors, Interleukin-12
MH  - Tumor Cells, Cultured
EDAT- 2002/01/05 10:00
MHDA- 2002/05/15 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/15 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1097/00002371-200111000-00007 [doi]
PST - ppublish
SO  - J Immunother. 2001 Nov-Dec;24(6):502-10. doi: 10.1097/00002371-200111000-00007.