PMID- 11761150
OWN - NLM
STAT- MEDLINE
DCOM- 20020507
LR  - 20190910
IS  - 0301-486X (Print)
IS  - 0301-486X (Linking)
VI  - 152
IP  - 2
DP  - 2001
TI  - Rapid method for the determination of ochratoxin A in urine by immunoaffinity 
      column clean-up and high-performance liquid chromatography.
PG  - 91-5
AB  - A rapid and accurate method to quantify ochratoxin A (OTA) at ppt (pg/ml) levels 
      in urine has been developed. The method uses commercial immunoaffinity columns 
      for clean-up and reversed phase high-performance liquid chromatography (HPLC) 
      with fluorescence detector for quantification of the toxin. Average recoveries of 
      OTA from human urine spiked at levels from 0.05 ng/ml to 1.0 ng/ml ranged from 
      88% to 93%, with relative standard deviations (RSDs) between 1% and 8%. Detection 
      limit was 0.005 ng/ml. Out of 41 human urine samples, 25 were found positive to 
      OTA with only one sample exceeding 0.05 ng/ml; the latter originated from a 
      patient affected by karyomegalic interstitial nephritis. The method can be used 
      as a rapid and non-invasive tool to assess human and animal exposure to OTA in 
      epidemiological studies and to establish the possible role of OTA in acute animal 
      intoxications or human end-stage renal diseases.
FAU - Pascale, M
AU  - Pascale M
AD  - Istituto Tossine e Micotossine da Parassiti Vegetali, CNR, Bari, Italy. 
      itmpmp21@area.ba.cnr.it
FAU - Visconti, A
AU  - Visconti A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (Mycotoxins)
RN  - 0 (Ochratoxins)
RN  - 1779SX6LUY (ochratoxin A)
SB  - IM
MH  - Animals
MH  - Chromatography, Affinity/*methods
MH  - Chromatography, High Pressure Liquid
MH  - Humans
MH  - Mycotoxins/*urine
MH  - Ochratoxins/*urine
MH  - Rats
EDAT- 2002/01/05 10:00
MHDA- 2002/05/08 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/08 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1023/a:1012463227948 [doi]
PST - ppublish
SO  - Mycopathologia. 2001;152(2):91-5. doi: 10.1023/a:1012463227948.