PMID- 11761177
OWN - NLM
STAT- MEDLINE
DCOM- 20020514
LR  - 20190815
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 23
IP  - 2
DP  - 2002 Jan
TI  - Increasing hydroxyapatite incorporation into poly(methylmethacrylate) cement 
      increases osteoblast adhesion and response.
PG  - 569-76
AB  - Poly(methylmethacrylate) (PMMA) is the current standard for cement held 
      prostheses. It forms a strong bond with the implant, but the bond between the 
      cement and the bone is considered to be weak, with fibroblastic cells observed at 
      the implant site, rather than direct bone contact, a contributing factor leading 
      to implant failure. Incorporation of hydroxyapatite (HA) increases the biological 
      response to the cement from tissue around the implant site, thus giving increased 
      bone apposition. In this study, PMMA discs with 0, 4.6 and 8.8 vol%. HA were 
      examined. Primary human osteoblast-like cells (HOBs) were used for the biological 
      evaluation of the response to the cements in vitro. Morphology was observed using 
      scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). 
      Measurement of tritiated thymidine (3H-TdR) incorporation and alkaline 
      phosphatase (ALP) activity were used to assess proliferation and differentiation. 
      A synergy between increasing focal contact formation, cytoskeletal organisation, 
      cell proliferation and expression of phenotype was observed with increasing HA 
      volume. Preferential anchorage of HOBs to HA rather than PMMA was a prominent 
      observation.
FAU - Dalby, M J
AU  - Dalby MJ
AD  - IRC in Biomedical Materials, Institute of Orthopaedics, Stanmore, Middlesex, UK. 
      m.dalby@bio.gla.ac.uk
FAU - Di Silvio, L
AU  - Di Silvio L
FAU - Harper, E J
AU  - Harper EJ
FAU - Bonfield, W
AU  - Bonfield W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Bone Cements)
RN  - 9011-14-7 (Polymethyl Methacrylate)
RN  - 91D9GV0Z28 (Durapatite)
SB  - IM
MH  - *Bone Cements
MH  - *Cell Adhesion
MH  - Cell Line
MH  - Durapatite/*chemistry
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts/*cytology/ultrastructure
MH  - Polymethyl Methacrylate/*chemistry
EDAT- 2002/01/05 10:00
MHDA- 2002/05/15 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/15 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - S0142-9612(01)00139-9 [pii]
AID - 10.1016/s0142-9612(01)00139-9 [doi]
PST - ppublish
SO  - Biomaterials. 2002 Jan;23(2):569-76. doi: 10.1016/s0142-9612(01)00139-9.