PMID- 11761440
OWN - NLM
STAT- MEDLINE
DCOM- 20020108
LR  - 20240426
IS  - 0340-7004 (Print)
IS  - 1432-0851 (Electronic)
IS  - 0340-7004 (Linking)
VI  - 50
IP  - 9
DP  - 2001 Nov
TI  - Dendritic cells pulsed with tumor extract-cationic liposome complex increase the 
      induction of cytotoxic T lymphocytes in mouse brain tumor.
PG  - 463-8
AB  - Dendritic cells (DCs) are professional antigen-presenting cells (APCs) that 
      locate in peripheral organs. It has been thought that a systemic immune response 
      does not play a role in regression of central nervous system (CNS) tumors, 
      because the CNS is an immunologically privileged site. However, recent advances 
      in immunology have led to the possibility of immunotherapy using peripheral DCs 
      against CNS tumors. Here, we investigated whether DCs pulsed with tumor extract 
      could induce an antitumor effect against malignant glioma. Furthermore, we also 
      investigated whether the antitumor effect become higher by pulsation with tumor 
      extract-liposome complex, compared to pulsation with tumor extract alone. As a 
      liposome, we used cationic small unilamellar vesicles composed of 
      N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), 
      dilauroylphosphatidylcholine (DLPC), and dioleoylphosphatidylethanolamine (DOPE) 
      in a molar ratio of 1:2:2. After intracerebral inoculation of mouse malignant 
      glioma GL261 cells into syngeneic C57BL/6 mice, DCs pulsed with extract from the 
      glioma cells by sonication were administered intraperitoneally thrice weekly on 
      days 7, 14 and 21. Tumor growth inhibition was evaluated by measuring the tumor 
      size 1 month after the tumor inoculation. The group treated with DCs pulsed by 
      tumor extract was inhibited in tumor progression compared with the control 
      non-pulsed DCs group, and the group treated with DCs pulsed by tumor extract and 
      liposomes showed substantial tumor volume reductions in all the mice. Among the 
      mice, there were several with no visible masses in their brains. 
      Immunohistochemical study showed that the CD8-positive cytotoxic T cells (CTLs) 
      were strongly recognized among the almost disappearing tumor cells of pulsed DCs 
      groups. The CTLs showed a specific antitumor activity for GL261 mouse glioma 
      cells. These findings indicated that DCs pulsed with tumor extract and liposomes 
      might play an important role in the activation of an immune response in malignant 
      glioma.
FAU - Aoki, H
AU  - Aoki H
AD  - Department of Neurosurgery, Nagoya University Graduate School of Medicine, Japan.
FAU - Mizuno, M
AU  - Mizuno M
FAU - Natsume, A
AU  - Natsume A
FAU - Tsugawa, T
AU  - Tsugawa T
FAU - Tsujimura, K
AU  - Tsujimura K
FAU - Takahashi, T
AU  - Takahashi T
FAU - Yoshida, J
AU  - Yoshida J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Cancer Immunol Immunother
JT  - Cancer immunology, immunotherapy : CII
JID - 8605732
RN  - 0 (Liposomes)
SB  - IM
MH  - Animals
MH  - Brain Neoplasms/*immunology/prevention & control
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/drug effects/*immunology
MH  - Female
MH  - Glioma/*immunology/prevention & control
MH  - Immunoenzyme Techniques
MH  - Immunotherapy
MH  - Liposomes
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Vaccination
PMC - PMC11032760
EDAT- 2002/01/05 10:00
MHDA- 2002/01/10 10:01
PMCR- 2001/10/18
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/01/10 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PHST- 2001/10/18 00:00 [pmc-release]
AID - 00220 [pii]
AID - 10.1007/s002620100220 [doi]
PST - ppublish
SO  - Cancer Immunol Immunother. 2001 Nov;50(9):463-8. doi: 10.1007/s002620100220.