PMID- 11763127
OWN - NLM
STAT- MEDLINE
DCOM- 20020603
LR  - 20221207
IS  - 0894-0282 (Print)
IS  - 0894-0282 (Linking)
VI  - 14
IP  - 11
DP  - 2001 Nov
TI  - The role of G protein alpha subunits in the infection process of the gray mold 
      fungus Botrytis cinerea.
PG  - 1293-302
AB  - To identify signal transduction pathways of the gray mold fungus Botrytis cinerea 
      involved in host infection, we used heterologous hybridization and a polymerase 
      chain reaction (PCR)-based approach to isolate two genes (bcg1 and bcg2) encoding 
      alpha subunits of heterotrimeric GTP-binding proteins. Both genes have homologues 
      in other fungi: bcg1 is a member of the G alpha(i) class, whereas bcg2 has 
      similarities to the magC gene of Magnaporthe grisea and the gna-2 gene of 
      Neurospora crassa. Reverse-transcription (RT)-PCR experiments showed clearly that 
      both genes are expressed at very early stages in infected bean leaves. Gene 
      replacement experiments were performed for both genes. bcg1 null mutants differ 
      in colony morphology from the wild-type strain, do not secrete extracellular 
      proteases, and show clearly reduced pathogenicity on bean and tomato. Conidia 
      germination and penetration of plant tissue is not disturbed in bcg1 mutants, but 
      the infection process stops after formation of primary lesions. In contrast, bcg2 
      mutants show wild-type colony morphology in axenic culture and are only slightly 
      reduced in pathogenicity. Complementation of bcg1 mutants with the wild-type gene 
      copy led to the full recovery of colony morphology, protease secretion, and 
      pathogenicity on both host plants. Application of exogenous cyclic AMP restored 
      the wild-type growth pattern of bcg1 mutants, but not the protease secretion, 
      implicating an essential role of BCG1 in different signaling pathways.
FAU - Gronover, C S
AU  - Gronover CS
AD  - Westfalische Wilhelms-Universitat Munster, Institut fur Botanik, Germany.
FAU - Kasulke, D
AU  - Kasulke D
FAU - Tudzynski, P
AU  - Tudzynski P
FAU - Tudzynski, B
AU  - Tudzynski B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Plant Microbe Interact
JT  - Molecular plant-microbe interactions : MPMI
JID - 9107902
RN  - EC 3.6.5.1 (Heterotrimeric GTP-Binding Proteins)
SB  - IM
MH  - Botrytis/genetics/*metabolism/*pathogenicity
MH  - Cloning, Molecular
MH  - Fabaceae/growth & development/*microbiology
MH  - Gene Expression Regulation, Developmental
MH  - Gene Targeting
MH  - Genes, Fungal
MH  - Genetic Complementation Test
MH  - Heterotrimeric GTP-Binding Proteins/genetics/*metabolism
MH  - Solanum lycopersicum/growth & development/microbiology
MH  - Mutation
MH  - Plant Diseases/microbiology
MH  - Signal Transduction
EDAT- 2002/01/05 10:00
MHDA- 2002/06/04 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/06/04 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1094/MPMI.2001.14.11.1293 [doi]
PST - ppublish
SO  - Mol Plant Microbe Interact. 2001 Nov;14(11):1293-302. doi: 
      10.1094/MPMI.2001.14.11.1293.