PMID- 11763176
OWN - NLM
STAT- MEDLINE
DCOM- 20020510
LR  - 20190916
IS  - 0002-9645 (Print)
IS  - 0002-9645 (Linking)
VI  - 62
IP  - 12
DP  - 2001 Dec
TI  - Anticoagulant effects of repeated subcutaneous injections of high doses of 
      unfractionated heparin in healthy dogs.
PG  - 1887-91
AB  - OBJECTIVE: To evaluate s.c. administration of unfractionated heparin (UFH) in 
      accordance with a dosing regimen for high-dose treatment in dogs. ANIMALS: 10 
      healthy adult Beagles. PROCEDURES: Two groups of dogs (5 dogs/group) were given 6 
      injections of heparin (500 units of UFH/kg of body weight, s.c.) at intervals of 
      8 (experiment 1) and 12 (experiment 2) hours. Blood samples were collected before 
      and 4 hours after heparin injections to determine amidolytic heparin activity, 
      activated partial thromboplastin time (APTT), thrombin time, antithrombin 
      activity, platelet count, and Hct. RESULTS: For experiments 1 and 2, mean +/- SD 
      heparin activities before (experiment 1, 1.32 +/- 0.20 U/ml; experiment 2, 0.69 
      +/- 0.174 U/ml) and 4 hours after the last heparin injection (experiment 1, 1.71 
      +/- 0.30 U/ml; experiment 2, 1.10 +/- 0.30 U/ml) were higher than values 
      calculated for the regimen used in experiment 1. Results of the investigated 
      thrombin time test system with low thrombin activity were frequently beyond the 
      measurement range, even with UFH activities > or = 0.6 U/ml. Moreover, a severe 
      decrease of antithrombin activity became evident during both experiments (eg, in 
      experiment 2 from 95.6 +/- 4.8 to 59.2 +/- 6.6%). In each treatment group, 2 dogs 
      developed hematomas. CONCLUSIONS AND CLINICAL RELEVANCE: Calculations of the 
      course of heparin activity after a single injection do not result in a reliable 
      dosing regimen for high-dose heparin treatment in dogs. High-dose treatment must 
      be monitored for each dog. Thrombin time measured with low thrombin activity is 
      unsuitable for this purpose.
FAU - Mischke, R H
AU  - Mischke RH
AD  - Clinic for Small Animals, School of Veterinary Medicine, Hannover, Germany.
FAU - Schuttert, C
AU  - Schuttert C
FAU - Grebe, S I
AU  - Grebe SI
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Vet Res
JT  - American journal of veterinary research
JID - 0375011
RN  - 0 (Anticoagulants)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - Anticoagulants/administration & dosage/blood/*pharmacology
MH  - Dogs/*blood
MH  - Female
MH  - Hematocrit/veterinary
MH  - Heparin/administration & dosage/blood/*pharmacology
MH  - Injections, Subcutaneous/veterinary
MH  - Male
MH  - Partial Thromboplastin Time/veterinary
MH  - Platelet Count/veterinary
MH  - Statistics, Nonparametric
MH  - Thrombin Time/veterinary
EDAT- 2002/01/05 10:00
MHDA- 2002/05/11 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/11 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.2460/ajvr.2001.62.1887 [doi]
PST - ppublish
SO  - Am J Vet Res. 2001 Dec;62(12):1887-91. doi: 10.2460/ajvr.2001.62.1887.