PMID- 11763310
OWN - NLM
STAT- MEDLINE
DCOM- 20020502
LR  - 20191105
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 10
IP  - 4
DP  - 2001 Dec
TI  - Detection of t(I 1; 18) in MALT-type lymphoma with dual-color fluorescence in 
      situ hybridization and reverse transcriptase-polymerase chain reaction analysis.
PG  - 207-13
AB  - t(11:8) is a recurrent chromosomal abnormality observed in mucosa-associated 
      lymphoid tissue (MALT)-type lymphoma. API2 and MLT genes have been implicated. 
      The authors devised a dual-color interphase fluorescence in situ hybridization 
      (FISH) system to detect splitting of 11q22 and its fusion with 18q21. Subjects 
      were 44 cases of extranodal lymphoma and cases of primary macroglobulinemia. 
      Whenever RNA was available, reverse transcriptase-polymerase chain reaction 
      followed by sequence analysis was performed. Positive cases by dual-color FISH 
      analysis were restricted to MALT-type lymphoma and one case of primary 
      macroglobulinemia. Among 24 cases of MALT-type lymphoma, 14 (58%) (4 gastric, 5 
      pulmonary, 3 orbital, 1 salivary, and 1 thyroid lymphomas) had splitting of the 
      11q22 region probes and fusion of signals suggesting the translocation of 
      chromosome 11 and 18. Reverse transcriptase-polymerase chain reaction analysis 
      showed the API2/MLT gene fusion in 9 of 10 cases. Sequence analyses showed three 
      different modes of involvement of the MLT gene, whereas the breakpoint at API2 
      was the same. Monoclonal component of serum immunoglobulin M was observed in 3 of 
      14 positive cases for the translocation. Direct visualization using dual-color 
      FISH on samples serves as a molecular tool for management of MALT-type lymphoma 
      with API2/MLT gene fusion.
FAU - Kobayashi, Y
AU  - Kobayashi Y
AD  - Endoscopy Division, National Cancer Center Hospital, Tokyo, Japan. 
      ykkobaya@gan2.ncc.go.jp
FAU - Nakata, M
AU  - Nakata M
FAU - Maekawa, M
AU  - Maekawa M
FAU - Takahashi, M
AU  - Takahashi M
FAU - Fujii, H
AU  - Fujii H
FAU - Matsuno, Y
AU  - Matsuno Y
FAU - Fujishiro, M
AU  - Fujishiro M
FAU - Ono, H
AU  - Ono H
FAU - Saito, D
AU  - Saito D
FAU - Takenaka, T
AU  - Takenaka T
FAU - Hirase, N
AU  - Hirase N
FAU - Nishimura, J
AU  - Nishimura J
FAU - Akioka, T
AU  - Akioka T
FAU - Enomoto, K
AU  - Enomoto K
FAU - Mikuni, C
AU  - Mikuni C
FAU - Hishima, T
AU  - Hishima T
FAU - Fukayama, M
AU  - Fukayama M
FAU - Sugano, K
AU  - Sugano K
FAU - Hosoda, F
AU  - Hosoda F
FAU - Ohki, M
AU  - Ohki M
FAU - Tobinai, K
AU  - Tobinai K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.22.- (MALT1 protein, human)
RN  - EC 3.4.22.- (Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Caspases
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Inhibitor of Apoptosis Proteins
MH  - Lymphoma, B-Cell, Marginal Zone/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
MH  - Neoplasm Proteins/genetics
MH  - Proteins/genetics
MH  - RNA, Messenger/metabolism
MH  - RNA, Neoplasm/analysis
MH  - Recombinant Fusion Proteins/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Translocation, Genetic
EDAT- 2002/01/05 10:00
MHDA- 2002/05/03 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/03 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1097/00019606-200112000-00001 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2001 Dec;10(4):207-13. doi: 10.1097/00019606-200112000-00001.