PMID- 11763311
OWN - NLM
STAT- MEDLINE
DCOM- 20020502
LR  - 20191210
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 10
IP  - 4
DP  - 2001 Dec
TI  - Use of novel t(11;14) and t(14;18) dual-fusion fluorescence in situ hybridization 
      probes in the differential diagnosis of lymphomas of small lymphocytes.
PG  - 214-22
AB  - Increasingly, molecular biologic techniques have become important in the 
      diagnosis of non-Hodgkin lymphomas. In the differential diagnosis of lymphoma(s) 
      of small lymphocytes (LSL), reliable detection of t(11;14) or t(14;18) would 
      confirm the diagnosis of mantle cell lymphoma (MCL) or follicle center lymphoma 
      (FCL), respectively. A total of 87 LSL cases (27 MCL, 39 FCL, 17 small 
      lymphocytic lymphoma [SLL], 3 marginal zone lymphomas, and 1 paraimmunoblastic 
      variant of SLL) were diagnosed by a combination of light microscopy, 
      immunohistochemistry, and flow cytometric immunophenotyping. Interphase 
      fluorescence in situ hybridization (FISH) for t(11;14) and t( 14;18) using 
      dual-fusion probes (Vysis, Downers Grove, IL) was performed on touch (n = 69) or 
      gravity (n = 18) preparations from these cases. Of 27 MCL cases tested, 25 (93%) 
      had demonstrable t(11;14), none had t(14;18), and 2 were negative for t(11;14) 
      and t(14;18). Twenty-five of 39 (64%) FCL cases had t(14;18), none had t(11;14), 
      and the remaining FCL cases (14 cases [35%]) had neither t(11;14) nor t(14;18). 
      All 17 (100%) SLL cases had neither t(11;14) nor t(14;18). All 3 (100%) marginal 
      zone lymphoma cases had neither t(11;14) nor t(14;18). The case of 
      paraimmunoblastic variant of SLL had t(11;14) and was negative for t(14;18). No 
      discrepant [i.e., positive for both t(11;14) and t(14;18)] or false-positive 
      cases were noted. Interphase FISH using these commercially available probes is a 
      useful adjunct to light microscopy, immunohistochemistry, and flow cytometric 
      immunophenotyping in the diagnosis of LSL. FISH can be performed successfully on 
      archival single-cell preparations (touch preparations or gravity preparations) 
      when fresh tissue is unavailable. No discordant or false-positive cases were 
      identified.
FAU - Frater, J L
AU  - Frater JL
AD  - Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 44195, USA.
FAU - Tsiftsakis, E K
AU  - Tsiftsakis EK
FAU - Hsi, E D
AU  - Hsi ED
FAU - Pettay, J
AU  - Pettay J
FAU - Tubbs, R R
AU  - Tubbs RR
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 14
MH  - *Chromosomes, Human, Pair 18
MH  - DNA Probes
MH  - DNA, Neoplasm/analysis
MH  - Diagnosis, Differential
MH  - Flow Cytometry
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/classification/*genetics/pathology
MH  - Reproducibility of Results
MH  - Retrospective Studies
MH  - *Translocation, Genetic
EDAT- 2002/01/05 10:00
MHDA- 2002/05/03 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/05/03 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
AID - 10.1097/00019606-200112000-00002 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2001 Dec;10(4):214-22. doi: 10.1097/00019606-200112000-00002.