PMID- 11764160
OWN - NLM
STAT- MEDLINE
DCOM- 20020328
LR  - 20161124
IS  - 0730-7268 (Print)
IS  - 0730-7268 (Linking)
VI  - 20
IP  - 12
DP  - 2001 Dec
TI  - Characterization of dioxin-like activity of sediments from a Czech river basin.
PG  - 2768-77
AB  - Synthetic organic chemicals are present in environmental compartments as complex 
      mixtures and therefore their potential effects are difficult to predict. In this 
      study, in vitro bioassays using wild-type fish and rat hepatoma cell lines and 
      their corresponding recombinant cell systems were used to evaluate 
      2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-like activity in extracts of sediments 
      collected from rivers of the Czech Republic. All the sediment extracts elicited 
      statistically significant responses in all the cell lines tested. For most 
      sediment extracts, a complete dose-response relationship was obtained. The 
      maximal efficacy of the samples was between 57 and 143% of the maximal induction 
      elicited by TCDD. Greater responsiveness, sensitivity, and reproducibility were 
      observed for recombinant than wild-type cells. Cell line-specific differences in 
      the sensitivity to compounds present in the complex sediment extracts were 
      observed. The TCDD equivalents (TCDD-EQs) determined from the different cell 
      bioassays were correlated. Greater concentrations of TCDD-EQs were obtained with 
      fish cell lines. The TCDD-EQs calculated from the results of chemical analysis of 
      toxic equivalents (TEQs) were in good agreement with those determined by 
      bioassays; the arly hydrocaron receptor (AhR)-effects of the identified chemicals 
      appear to be generally additive. This indicates that most of the TCDD-like 
      activity was accounted for by the compounds identified and quantified by 
      instrumental analysis. Fractionation along with mass-balance calculations allowed 
      identification of the active fractions and classes of compounds. Polycyclic 
      aromatic hydrocarbons (PAHs) were found to be responsible for most of the 
      AhR-mediated activity in sediments.
FAU - Hilscherova, K
AU  - Hilscherova K
AD  - Department of Environmental Chemistry and Ecotoxicology, Faculty of Science, 
      Masaryk University, Brno, Czech Republic. klara@chemi.muni.cz
FAU - Kannan, K
AU  - Kannan K
FAU - Kang, Y S
AU  - Kang YS
FAU - Holoubek, I
AU  - Holoubek I
FAU - Machala, M
AU  - Machala M
FAU - Masunaga, S
AU  - Masunaga S
FAU - Nakanishi, J
AU  - Nakanishi J
FAU - Giesy, J P
AU  - Giesy JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Toxicol Chem
JT  - Environmental toxicology and chemistry
JID - 8308958
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - Biological Assay
MH  - Carcinoma, Hepatocellular/pathology
MH  - Dose-Response Relationship, Drug
MH  - Environmental Monitoring
MH  - Environmental Pollutants/*adverse effects/analysis
MH  - Fishes
MH  - Geologic Sediments/*chemistry
MH  - Liver Neoplasms/pathology
MH  - Polychlorinated Dibenzodioxins/*adverse effects/*analogs & derivatives/analysis
MH  - Polycyclic Aromatic Hydrocarbons/adverse effects/analysis
MH  - Rats
MH  - Reproducibility of Results
MH  - Tumor Cells, Cultured
MH  - Water Pollutants, Chemical/*analysis
EDAT- 2002/01/05 10:00
MHDA- 2002/03/29 10:01
CRDT- 2002/01/05 10:00
PHST- 2002/01/05 10:00 [pubmed]
PHST- 2002/03/29 10:01 [medline]
PHST- 2002/01/05 10:00 [entrez]
PST - ppublish
SO  - Environ Toxicol Chem. 2001 Dec;20(12):2768-77.